2ac1
From Proteopedia
(New page: 200px<br /><applet load="2ac1" size="350" color="white" frame="true" align="right" spinBox="true" caption="2ac1, resolution 2.15Å" /> '''Crystal structure of...) |
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==Overview== | ==Overview== | ||
| - | Cell-wall invertases play crucial roles during plant development. They | + | Cell-wall invertases play crucial roles during plant development. They hydrolyse sucrose into its fructose and glucose subunits by cleavage of the alpha1-beta2 glycosidic bond. Here, the structure of the Arabidopsis thaliana cell-wall invertase 1 (AtcwINV1; gene accession code At3g13790) is described at a resolution of 2.15 A. The structure comprises an N-terminal fivefold beta-propeller domain followed by a C-terminal domain formed by two beta-sheets. The active site is positioned in the fivefold beta-propeller domain, containing the nucleophile Asp23 and the acid/base catalyst Glu203 of the double-displacement enzymatic reaction. The function of the C-terminal domain remains unknown. Unlike in other GH 32 family enzyme structures known to date, in AtcwINV1 the cleft formed between both domains is blocked by Asn299-linked carbohydrates. A preliminary site-directed mutagenesis experiment (Asn299Asp) removed the glycosyl chain but did not alter the activity profile of the enzyme. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Beta-fructofuranosidase]] | [[Category: Beta-fructofuranosidase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Ende, W | + | [[Category: Ende, W Van den.]] |
| - | [[Category: Laere, A | + | [[Category: Laere, A Van.]] |
[[Category: Rabijns, A.]] | [[Category: Rabijns, A.]] | ||
| - | [[Category: Ranter, C | + | [[Category: Ranter, C De.]] |
| - | [[Category: Roy, K | + | [[Category: Roy, K Le.]] |
[[Category: Verhaest, M.]] | [[Category: Verhaest, M.]] | ||
[[Category: GOL]] | [[Category: GOL]] | ||
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[[Category: five fold beta propeller]] | [[Category: five fold beta propeller]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:25:55 2008'' |
Revision as of 14:26, 21 February 2008
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Crystal structure of a cell-wall invertase from Arabidopsis thaliana
Overview
Cell-wall invertases play crucial roles during plant development. They hydrolyse sucrose into its fructose and glucose subunits by cleavage of the alpha1-beta2 glycosidic bond. Here, the structure of the Arabidopsis thaliana cell-wall invertase 1 (AtcwINV1; gene accession code At3g13790) is described at a resolution of 2.15 A. The structure comprises an N-terminal fivefold beta-propeller domain followed by a C-terminal domain formed by two beta-sheets. The active site is positioned in the fivefold beta-propeller domain, containing the nucleophile Asp23 and the acid/base catalyst Glu203 of the double-displacement enzymatic reaction. The function of the C-terminal domain remains unknown. Unlike in other GH 32 family enzyme structures known to date, in AtcwINV1 the cleft formed between both domains is blocked by Asn299-linked carbohydrates. A preliminary site-directed mutagenesis experiment (Asn299Asp) removed the glycosyl chain but did not alter the activity profile of the enzyme.
About this Structure
2AC1 is a Single protein structure of sequence from Arabidopsis thaliana with and as ligands. Active as Beta-fructofuranosidase, with EC number 3.2.1.26 Full crystallographic information is available from OCA.
Reference
X-ray diffraction structure of a cell-wall invertase from Arabidopsis thaliana., Verhaest M, Lammens W, Le Roy K, De Coninck B, De Ranter CJ, Van Laere A, Van den Ende W, Rabijns A, Acta Crystallogr D Biol Crystallogr. 2006 Dec;62(Pt 12):1555-63. Epub 2006, Nov 23. PMID:17139091
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