2adt
From Proteopedia
(New page: 200px<br /><applet load="2adt" size="350" color="white" frame="true" align="right" spinBox="true" caption="2adt" /> '''NMR structure of a 30 kDa GAAA tetraloop-rec...) |
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==Overview== | ==Overview== | ||
| - | Tertiary interactions are critical for proper RNA folding and ribozyme | + | Tertiary interactions are critical for proper RNA folding and ribozyme catalysis. RNA tertiary structure is often condensed through long-range helical packing interactions mediated by loop-receptor motifs. RNA structures displaying helical packing by loop-receptor interactions have been solved by X-ray crystallography, but not by NMR. Here, we report the NMR structure of a 30 kDa GAAA tetraloop-receptor RNA complex. In order to stabilize the complex, we used a modular design in which the RNA was engineered to form a homodimer, with each subunit containing a GAAA tetraloop phased one helical turn apart from its cognate 11-nucleotide receptor domain. The structure determination utilized specific isotopic labeling patterns (2H, 13C and 15N) and refinement against residual dipolar couplings. We observe a unique and highly unusual chemical shift pattern for an adenosine platform interaction that reveals a spectroscopic fingerprint for this motif. The structure of the GAAA tetraloop-receptor interaction is well defined solely from experimental NMR data, shows minor deviations from previously solved crystal structures, and verifies the previously inferred hydrogen bonding patterns within this motif. This work demonstrates the feasibility of using engineered homodimers as modular systems for the determination of RNA tertiary interactions by NMR. |
==About this Structure== | ==About this Structure== | ||
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RNA helical packing in solution: NMR structure of a 30 kDa GAAA tetraloop-receptor complex., Davis JH, Tonelli M, Scott LG, Jaeger L, Williamson JR, Butcher SE, J Mol Biol. 2005 Aug 12;351(2):371-82. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16002091 16002091] | RNA helical packing in solution: NMR structure of a 30 kDa GAAA tetraloop-receptor complex., Davis JH, Tonelli M, Scott LG, Jaeger L, Williamson JR, Butcher SE, J Mol Biol. 2005 Aug 12;351(2):371-82. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16002091 16002091] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Butcher, S | + | [[Category: Butcher, S E.]] |
| - | [[Category: Davis, J | + | [[Category: Davis, J H.]] |
[[Category: Jaeger, L.]] | [[Category: Jaeger, L.]] | ||
| - | [[Category: Scott, L | + | [[Category: Scott, L G.]] |
[[Category: Tonelli, M.]] | [[Category: Tonelli, M.]] | ||
| - | [[Category: Williamson, J | + | [[Category: Williamson, J R.]] |
[[Category: gaaa tetraloop gaaa tetraloop-receptor]] | [[Category: gaaa tetraloop gaaa tetraloop-receptor]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:26:20 2008'' |
Revision as of 14:26, 21 February 2008
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NMR structure of a 30 kDa GAAA tetraloop-receptor complex.
Overview
Tertiary interactions are critical for proper RNA folding and ribozyme catalysis. RNA tertiary structure is often condensed through long-range helical packing interactions mediated by loop-receptor motifs. RNA structures displaying helical packing by loop-receptor interactions have been solved by X-ray crystallography, but not by NMR. Here, we report the NMR structure of a 30 kDa GAAA tetraloop-receptor RNA complex. In order to stabilize the complex, we used a modular design in which the RNA was engineered to form a homodimer, with each subunit containing a GAAA tetraloop phased one helical turn apart from its cognate 11-nucleotide receptor domain. The structure determination utilized specific isotopic labeling patterns (2H, 13C and 15N) and refinement against residual dipolar couplings. We observe a unique and highly unusual chemical shift pattern for an adenosine platform interaction that reveals a spectroscopic fingerprint for this motif. The structure of the GAAA tetraloop-receptor interaction is well defined solely from experimental NMR data, shows minor deviations from previously solved crystal structures, and verifies the previously inferred hydrogen bonding patterns within this motif. This work demonstrates the feasibility of using engineered homodimers as modular systems for the determination of RNA tertiary interactions by NMR.
About this Structure
2ADT is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
RNA helical packing in solution: NMR structure of a 30 kDa GAAA tetraloop-receptor complex., Davis JH, Tonelli M, Scott LG, Jaeger L, Williamson JR, Butcher SE, J Mol Biol. 2005 Aug 12;351(2):371-82. PMID:16002091
Page seeded by OCA on Thu Feb 21 16:26:20 2008
