2bps
From Proteopedia
(New page: 200px<br /><applet load="2bps" size="350" color="white" frame="true" align="right" spinBox="true" caption="2bps, resolution 2.7Å" /> '''UBIQUITIN-LIKE PROTEI...) |
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==Overview== | ==Overview== | ||
| - | The YukD protein in Bacillus subtilis was identified in a hidden Markov | + | The YukD protein in Bacillus subtilis was identified in a hidden Markov model (HMM) search as being related in sequence to ubiquitin. By solving the crystal structure we show that YukD adopts a fold that is most closely related to ubiquitin, yet has the shortest C-terminal tail of all known ubiquitin-like proteins. The endogenous gene of yukD in B. subtilis was disrupted without an obvious phenotypic effect and an inducible copy encoding a C-Myc and His-tagged version of the protein was introduced at the ectopic locus amyE. Conjugation assays performed both in vitro and in vivo indicate that YukD lacks the capacity for covalent bond formation with other proteins. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Bacillus subtilis]] | [[Category: Bacillus subtilis]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Ent, F | + | [[Category: Ent, F Van Den.]] |
[[Category: Lowe, J.]] | [[Category: Lowe, J.]] | ||
[[Category: ubiquitin]] | [[Category: ubiquitin]] | ||
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[[Category: yukd]] | [[Category: yukd]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:40:17 2008'' |
Revision as of 14:40, 21 February 2008
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UBIQUITIN-LIKE PROTEIN YUKD OF BACILLUS SUBTILIS
Overview
The YukD protein in Bacillus subtilis was identified in a hidden Markov model (HMM) search as being related in sequence to ubiquitin. By solving the crystal structure we show that YukD adopts a fold that is most closely related to ubiquitin, yet has the shortest C-terminal tail of all known ubiquitin-like proteins. The endogenous gene of yukD in B. subtilis was disrupted without an obvious phenotypic effect and an inducible copy encoding a C-Myc and His-tagged version of the protein was introduced at the ectopic locus amyE. Conjugation assays performed both in vitro and in vivo indicate that YukD lacks the capacity for covalent bond formation with other proteins.
About this Structure
2BPS is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.
Reference
Crystal structure of the ubiquitin-like protein YukD from Bacillus subtilis., van den Ent F, Lowe J, FEBS Lett. 2005 Jul 4;579(17):3837-41. PMID:15978580
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