2df7
From Proteopedia
(New page: 200px<br /><applet load="2df7" size="350" color="white" frame="true" align="right" spinBox="true" caption="2df7, resolution 2.6Å" /> '''Crystal structure of ...) |
|||
| Line 4: | Line 4: | ||
==Overview== | ==Overview== | ||
| - | The structural protein VP2 of infectious bursal disease virus (IBDV) | + | The structural protein VP2 of infectious bursal disease virus (IBDV) spontaneously forms a dodecahedral T=1 subviral particle (SVP), and is a primary immunogen of the virus. In this study, the structure of IBDV SVP was determined in a cubic crystal and refined to 2.6A resolution. It contains 20 independent VP2 subunits in a crystallographic asymmetric unit. Each subunit is folded mainly into a shell domain and a protrusion domain, both with the Swiss-roll topology, plus a small helical base domain. Three VP2 subunits constitute a tight trimer, which is the building block of IBDV (sub)viral particles. The structure revealed a calcium ion bound to three pairs of symmetry-related Asp31 and Asp174 to stabilize the VP2 trimer. Our results of treatment of SVP with EGTA, a Ca(2+)-chelating reagent, indicated that the metal-ion may be important not only in maintaining highly stable quaternary structure but also in regulating the swelling and dissociation of the icosahedral particles. A Ca(2+)-dependent assembly pathway was thus proposed, which involves further interactions between the trimers. The 20 independent subunits showed conformational variations, with the surface loops of the protrusion domain being the most diverse. These loops are targets of the neutralizing antibodies. Several common interactions between the surface loops were clearly observed, suggesting a possible major conformation of the immunogenic epitopes. |
==About this Structure== | ==About this Structure== | ||
| Line 13: | Line 13: | ||
[[Category: Infectious bursal disease virus]] | [[Category: Infectious bursal disease virus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Ko, T | + | [[Category: Ko, T P.]] |
| - | [[Category: Lee, C | + | [[Category: Lee, C C.]] |
| - | [[Category: Wang, A | + | [[Category: Wang, A H.]] |
| - | [[Category: Wang, M | + | [[Category: Wang, M Y.]] |
[[Category: CA]] | [[Category: CA]] | ||
[[Category: CL]] | [[Category: CL]] | ||
| Line 25: | Line 25: | ||
[[Category: surface loop]] | [[Category: surface loop]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:58:13 2008'' |
Revision as of 14:58, 21 February 2008
|
Crystal structure of infectious bursal disease virus VP2 subviral particle
Overview
The structural protein VP2 of infectious bursal disease virus (IBDV) spontaneously forms a dodecahedral T=1 subviral particle (SVP), and is a primary immunogen of the virus. In this study, the structure of IBDV SVP was determined in a cubic crystal and refined to 2.6A resolution. It contains 20 independent VP2 subunits in a crystallographic asymmetric unit. Each subunit is folded mainly into a shell domain and a protrusion domain, both with the Swiss-roll topology, plus a small helical base domain. Three VP2 subunits constitute a tight trimer, which is the building block of IBDV (sub)viral particles. The structure revealed a calcium ion bound to three pairs of symmetry-related Asp31 and Asp174 to stabilize the VP2 trimer. Our results of treatment of SVP with EGTA, a Ca(2+)-chelating reagent, indicated that the metal-ion may be important not only in maintaining highly stable quaternary structure but also in regulating the swelling and dissociation of the icosahedral particles. A Ca(2+)-dependent assembly pathway was thus proposed, which involves further interactions between the trimers. The 20 independent subunits showed conformational variations, with the surface loops of the protrusion domain being the most diverse. These loops are targets of the neutralizing antibodies. Several common interactions between the surface loops were clearly observed, suggesting a possible major conformation of the immunogenic epitopes.
About this Structure
2DF7 is a Single protein structure of sequence from Infectious bursal disease virus with and as ligands. Full crystallographic information is available from OCA.
Reference
Crystal structure of infectious bursal disease virus VP2 subviral particle at 2.6A resolution: implications in virion assembly and immunogenicity., Lee CC, Ko TP, Chou CC, Yoshimura M, Doong SR, Wang MY, Wang AH, J Struct Biol. 2006 Jul;155(1):74-86. Epub 2006 Mar 31. PMID:16677827
Page seeded by OCA on Thu Feb 21 16:58:13 2008
Categories: Infectious bursal disease virus | Single protein | Ko, T P. | Lee, C C. | Wang, A H. | Wang, M Y. | CA | CL | Calcium | Epitope | Icosahedral capsid | Immunogen | Surface loop
