3ab3
From Proteopedia
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{{STRUCTURE_3ab3| PDB=3ab3 | SCENE= }} | {{STRUCTURE_3ab3| PDB=3ab3 | SCENE= }} | ||
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===Crystal structure of p115RhoGEF RGS domain in complex with G alpha 13=== | ===Crystal structure of p115RhoGEF RGS domain in complex with G alpha 13=== | ||
| + | {{ABSTRACT_PUBMED_21507947}} | ||
| - | + | ==Function== | |
| - | + | [[http://www.uniprot.org/uniprot/GNAI3_MOUSE GNAI3_MOUSE]] Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. G(k) is the stimulatory G protein of receptor-regulated K(+) channels. The active GTP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division. [[http://www.uniprot.org/uniprot/ARHG1_HUMAN ARHG1_HUMAN]] Seems to play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13) subunits. Acts as GTPase-activating protein (GAP) for GNA12 and GNA13, and as guanine nucleotide exchange factor (GEF) for RhoA GTPase. Activated G alpha 13/GNA13 stimulates the RhoGEF activity through interaction with the RGS-like domain. This GEF activity is inhibited by binding to activated GNA12. Mediates angiotensin-2-induced RhoA activation.<ref>PMID:8810315</ref> <ref>PMID:9641915</ref> <ref>PMID:9641916</ref> <ref>PMID:20098430</ref> | |
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==About this Structure== | ==About this Structure== | ||
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==Reference== | ==Reference== | ||
| - | <ref group="xtra">PMID:021507947</ref><references group="xtra"/> | + | <ref group="xtra">PMID:021507947</ref><references group="xtra"/><references/> |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
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[[Category: Shirouzu, M.]] | [[Category: Shirouzu, M.]] | ||
[[Category: Yokoyama, S.]] | [[Category: Yokoyama, S.]] | ||
| + | [[Category: Gtp-binding]] | ||
| + | [[Category: Gtpase activation]] | ||
| + | [[Category: Guanine-nucleotide releasing factor]] | ||
| + | [[Category: Lipoprotein]] | ||
| + | [[Category: Membrane]] | ||
| + | [[Category: Nucleotide-binding]] | ||
| + | [[Category: Palmitate]] | ||
| + | [[Category: Phosphoprotein]] | ||
| + | [[Category: Protein complex]] | ||
| + | [[Category: Signal transduction]] | ||
| + | [[Category: Signaling protein-membrane protein complex]] | ||
| + | [[Category: Transducer]] | ||
Revision as of 23:27, 10 April 2013
Contents |
Crystal structure of p115RhoGEF RGS domain in complex with G alpha 13
Template:ABSTRACT PUBMED 21507947
Function
[GNAI3_MOUSE] Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. G(k) is the stimulatory G protein of receptor-regulated K(+) channels. The active GTP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division. [ARHG1_HUMAN] Seems to play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13) subunits. Acts as GTPase-activating protein (GAP) for GNA12 and GNA13, and as guanine nucleotide exchange factor (GEF) for RhoA GTPase. Activated G alpha 13/GNA13 stimulates the RhoGEF activity through interaction with the RGS-like domain. This GEF activity is inhibited by binding to activated GNA12. Mediates angiotensin-2-induced RhoA activation.[1] [2] [3] [4]
About this Structure
3ab3 is a 4 chain structure with sequence from Homo sapiens and Mus musculus. Full crystallographic information is available from OCA.
Reference
- Hajicek N, Kukimoto-Niino M, Mishima-Tsumagari C, Chow CR, Shirouzu M, Terada T, Patel M, Yokoyama S, Kozasa T. Identification of Critical Residues in G{alpha}13 for Stimulation of p115RhoGEF Activity and the Structure of the G{alpha}13-p115RhoGEF Regulator of G Protein Signaling Homology (RH) Domain Complex. J Biol Chem. 2011 Jun 10;286(23):20625-36. Epub 2011 Apr 20. PMID:21507947 doi:10.1074/jbc.M110.201392
- ↑ Hart MJ, Sharma S, elMasry N, Qiu RG, McCabe P, Polakis P, Bollag G. Identification of a novel guanine nucleotide exchange factor for the Rho GTPase. J Biol Chem. 1996 Oct 11;271(41):25452-8. PMID:8810315
- ↑ Kozasa T, Jiang X, Hart MJ, Sternweis PM, Singer WD, Gilman AG, Bollag G, Sternweis PC. p115 RhoGEF, a GTPase activating protein for Galpha12 and Galpha13. Science. 1998 Jun 26;280(5372):2109-11. PMID:9641915
- ↑ Hart MJ, Jiang X, Kozasa T, Roscoe W, Singer WD, Gilman AG, Sternweis PC, Bollag G. Direct stimulation of the guanine nucleotide exchange activity of p115 RhoGEF by Galpha13. Science. 1998 Jun 26;280(5372):2112-4. PMID:9641916
- ↑ Guilluy C, Bregeon J, Toumaniantz G, Rolli-Derkinderen M, Retailleau K, Loufrani L, Henrion D, Scalbert E, Bril A, Torres RM, Offermanns S, Pacaud P, Loirand G. The Rho exchange factor Arhgef1 mediates the effects of angiotensin II on vascular tone and blood pressure. Nat Med. 2010 Feb;16(2):183-90. doi: 10.1038/nm.2079. Epub 2010 Jan 24. PMID:20098430 doi:10.1038/nm.2079
Categories: Homo sapiens | Mus musculus | Kozasa, T. | Kukimoto-Niino, M. | Mishima, C. | Shirouzu, M. | Yokoyama, S. | Gtp-binding | Gtpase activation | Guanine-nucleotide releasing factor | Lipoprotein | Membrane | Nucleotide-binding | Palmitate | Phosphoprotein | Protein complex | Signal transduction | Signaling protein-membrane protein complex | Transducer
