2fqn
From Proteopedia
(New page: 200px<br /><applet load="2fqn" size="350" color="white" frame="true" align="right" spinBox="true" caption="2fqn, resolution 2.300Å" /> '''Crystal structure o...) |
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==Overview== | ==Overview== | ||
| - | The decoding A site of the small ribosomal subunit is an RNA molecular | + | The decoding A site of the small ribosomal subunit is an RNA molecular switch, which monitors codon-anticodon interactions to guarantee translation fidelity. We have solved the crystal structure of an RNA fragment containing two Homo sapiens cytoplasmic A sites. Each of the two A sites presents a different conformational state. In one state, adenines A1492 and A1493 are fully bulged-out with C1409 forming a wobble-like pair to A1491. In the second state, adenines A1492 and A1493 form non-Watson-Crick pairs with C1409 and G1408, respectively while A1491 bulges out. The first state of the eukaryotic A site is, thus, basically the same as in the bacterial A site with bulging A1492 and A1493. It is the state used for recognition of the codon/anticodon complex. On the contrary, the second state of the H.sapiens cytoplasmic A site is drastically different from any of those observed for the bacterial A site without bulging A1492 and A1493. |
==About this Structure== | ==About this Structure== | ||
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[[Category: rrna]] | [[Category: rrna]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:24:09 2008'' |
Revision as of 15:24, 21 February 2008
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Crystal structure of the Homo sapiens cytoplasmic ribosomal decoding A site
Overview
The decoding A site of the small ribosomal subunit is an RNA molecular switch, which monitors codon-anticodon interactions to guarantee translation fidelity. We have solved the crystal structure of an RNA fragment containing two Homo sapiens cytoplasmic A sites. Each of the two A sites presents a different conformational state. In one state, adenines A1492 and A1493 are fully bulged-out with C1409 forming a wobble-like pair to A1491. In the second state, adenines A1492 and A1493 form non-Watson-Crick pairs with C1409 and G1408, respectively while A1491 bulges out. The first state of the eukaryotic A site is, thus, basically the same as in the bacterial A site with bulging A1492 and A1493. It is the state used for recognition of the codon/anticodon complex. On the contrary, the second state of the H.sapiens cytoplasmic A site is drastically different from any of those observed for the bacterial A site without bulging A1492 and A1493.
About this Structure
2FQN is a Protein complex structure of sequences from [1] with and as ligands. Full crystallographic information is available from OCA.
Reference
Two conformational states in the crystal structure of the Homo sapiens cytoplasmic ribosomal decoding A site., Kondo J, Urzhumtsev A, Westhof E, Nucleic Acids Res. 2006 Feb 1;34(2):676-85. Print 2006. PMID:16452297
Page seeded by OCA on Thu Feb 21 17:24:09 2008
Categories: Protein complex | Kondo, J. | Urzhumtsev, A. | Westhof, E. | MG | NCO | A site | Decoding site | Homo sapiens cytoplasm | Rna duplex | Rrna
