2fx0
From Proteopedia
(New page: 200px<br /><applet load="2fx0" size="350" color="white" frame="true" align="right" spinBox="true" caption="2fx0, resolution 2.40Å" /> '''Crystal Structure of...) |
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==Overview== | ==Overview== | ||
| - | Production of Bacillus cereus and Bacillus anthracis toxins is controlled | + | Production of Bacillus cereus and Bacillus anthracis toxins is controlled by a number of transcriptional regulators. Here we report the crystal structure of B. cereus HlyIIR, a regulator of the gene encoding the pore-forming toxin hemolysin II. We show that HlyIIR forms a tight dimer with a fold and overall architecture similar to the TetR family of repressors. A remarkable feature of the structure is a large internal cavity with a volume of 550 A(3) suggesting that the activity of HlyIIR is modulated by binding of a ligand, which triggers the toxin production. Virtual ligand library screening shows that this pocket can accommodate compounds with molecular masses of up to 400-500 Da. Based on structural data and previous biochemical evidence, we propose a model for HlyIIR interaction with the DNA. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Bacillus cereus]] | [[Category: Bacillus cereus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Antson, A | + | [[Category: Antson, A A.]] |
| - | [[Category: Kovalevskiy, O | + | [[Category: Kovalevskiy, O V.]] |
| - | [[Category: Lebedev, A | + | [[Category: Lebedev, A A.]] |
| - | [[Category: Solonin, A | + | [[Category: Solonin, A S.]] |
[[Category: transcriptional regulator]] | [[Category: transcriptional regulator]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:25:57 2008'' |
Revision as of 15:26, 21 February 2008
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Crystal Structure of HlyIIR, a Hemolysin II transcriptional Regulator
Overview
Production of Bacillus cereus and Bacillus anthracis toxins is controlled by a number of transcriptional regulators. Here we report the crystal structure of B. cereus HlyIIR, a regulator of the gene encoding the pore-forming toxin hemolysin II. We show that HlyIIR forms a tight dimer with a fold and overall architecture similar to the TetR family of repressors. A remarkable feature of the structure is a large internal cavity with a volume of 550 A(3) suggesting that the activity of HlyIIR is modulated by binding of a ligand, which triggers the toxin production. Virtual ligand library screening shows that this pocket can accommodate compounds with molecular masses of up to 400-500 Da. Based on structural data and previous biochemical evidence, we propose a model for HlyIIR interaction with the DNA.
About this Structure
2FX0 is a Single protein structure of sequence from Bacillus cereus. Full crystallographic information is available from OCA.
Reference
Crystal structure of Bacillus cereus HlyIIR, a transcriptional regulator of the gene for pore-forming toxin hemolysin II., Kovalevskiy OV, Lebedev AA, Surin AK, Solonin AS, Antson AA, J Mol Biol. 2007 Jan 19;365(3):825-34. Epub 2006 Oct 26. PMID:17097673
Page seeded by OCA on Thu Feb 21 17:25:57 2008
