This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
2g5q
From Proteopedia
(New page: 200px<br /><applet load="2g5q" size="350" color="white" frame="true" align="right" spinBox="true" caption="2g5q, resolution 2.700Å" /> '''Crystal structures ...) |
|||
| Line 4: | Line 4: | ||
==Overview== | ==Overview== | ||
| - | Amikacin is the 4,6-linked aminoglycoside modified at position N1 of the | + | Amikacin is the 4,6-linked aminoglycoside modified at position N1 of the 2-deoxystreptamine ring (ring II) by the L-haba group. In the present study, the crystal structure of a complex between oligonucleotide containing the bacterial ribosomal A site and amikacin has been solved at 2.7 A resolution. Amikacin specifically binds to the A site in practically the same way as its parent compound kanamycin. In addition, the L-haba group interacts with the upper side of the A site through two direct contacts, O2*...H-N4(C1496) and N4*-H...O6(G1497). The present crystal structure shows how the introduction of the L-haba group on ring II of aminoglycoside is an effective mutation for obtaining a higher affinity to the bacterial A site. |
==About this Structure== | ==About this Structure== | ||
| Line 14: | Line 14: | ||
[[Category: Francois, B.]] | [[Category: Francois, B.]] | ||
[[Category: Kondo, J.]] | [[Category: Kondo, J.]] | ||
| - | [[Category: Murray, J | + | [[Category: Murray, J B.]] |
| - | [[Category: Russell, R | + | [[Category: Russell, R J.M.]] |
[[Category: Westhof, E.]] | [[Category: Westhof, E.]] | ||
[[Category: AKN]] | [[Category: AKN]] | ||
| Line 25: | Line 25: | ||
[[Category: x-ray analysis]] | [[Category: x-ray analysis]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:28:27 2008'' |
Revision as of 15:28, 21 February 2008
|
Crystal structures of the bacterial ribosomal decoding site complexed with amikacin.
Overview
Amikacin is the 4,6-linked aminoglycoside modified at position N1 of the 2-deoxystreptamine ring (ring II) by the L-haba group. In the present study, the crystal structure of a complex between oligonucleotide containing the bacterial ribosomal A site and amikacin has been solved at 2.7 A resolution. Amikacin specifically binds to the A site in practically the same way as its parent compound kanamycin. In addition, the L-haba group interacts with the upper side of the A site through two direct contacts, O2*...H-N4(C1496) and N4*-H...O6(G1497). The present crystal structure shows how the introduction of the L-haba group on ring II of aminoglycoside is an effective mutation for obtaining a higher affinity to the bacterial A site.
About this Structure
2G5Q is a Protein complex structure of sequences from [1] with as ligand. Full crystallographic information is available from OCA.
Reference
Crystal structure of the bacterial ribosomal decoding site complexed with amikacin containing the gamma-amino-alpha-hydroxybutyryl (haba) group., Kondo J, Francois B, Russell RJ, Murray JB, Westhof E, Biochimie. 2006 Aug;88(8):1027-31. Epub 2006 Jun 13. PMID:16806634
Page seeded by OCA on Thu Feb 21 17:28:27 2008
