2hua
From Proteopedia
(New page: 200px<br /><applet load="2hua" size="350" color="white" frame="true" align="right" spinBox="true" caption="2hua" /> '''Solution Structure of CSFV IRES Domain IIa''...) |
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==Overview== | ==Overview== | ||
| - | Internal ribosome entry site (IRES) RNAs from the hepatitis C virus (HCV) | + | Internal ribosome entry site (IRES) RNAs from the hepatitis C virus (HCV) and classical swine fever virus (CSFV) coordinate cap-independent assembly of eukaryotic 48S initiation complexes, consisting of the 40S ribosomal subunit, eukaryotic initiation factor (eIF) 3 and the eIF2/GTP/Met-tRNA(i)(Met) ternary complex. Here, we report that these IRESes also play a functional role during 80S ribosome assembly downstream of 48S complex formation, in promoting eIF5-induced GTP hydrolysis and eIF2/GDP release from the initiation complex. We show that this function is encoded in their independently folded IRES domain II and that it depends both on its characteristic bent conformation and two conserved RNA motifs, an apical hairpin loop and a loop E. Our data suggest a general mode of subunit joining in HCV and HCV-like IRESes. |
==About this Structure== | ==About this Structure== | ||
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HCV and CSFV IRES domain II mediate eIF2 release during 80S ribosome assembly., Locker N, Easton LE, Lukavsky PJ, EMBO J. 2007 Feb 7;26(3):795-805. Epub 2007 Jan 25. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17255934 17255934] | HCV and CSFV IRES domain II mediate eIF2 release during 80S ribosome assembly., Locker N, Easton LE, Lukavsky PJ, EMBO J. 2007 Feb 7;26(3):795-805. Epub 2007 Jan 25. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17255934 17255934] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Easton, L | + | [[Category: Easton, L E.]] |
[[Category: Locker, N.]] | [[Category: Locker, N.]] | ||
| - | [[Category: Lukavsky, P | + | [[Category: Lukavsky, P J.]] |
[[Category: rna hairpin]] | [[Category: rna hairpin]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:45:48 2008'' |
Revision as of 15:45, 21 February 2008
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Solution Structure of CSFV IRES Domain IIa
Overview
Internal ribosome entry site (IRES) RNAs from the hepatitis C virus (HCV) and classical swine fever virus (CSFV) coordinate cap-independent assembly of eukaryotic 48S initiation complexes, consisting of the 40S ribosomal subunit, eukaryotic initiation factor (eIF) 3 and the eIF2/GTP/Met-tRNA(i)(Met) ternary complex. Here, we report that these IRESes also play a functional role during 80S ribosome assembly downstream of 48S complex formation, in promoting eIF5-induced GTP hydrolysis and eIF2/GDP release from the initiation complex. We show that this function is encoded in their independently folded IRES domain II and that it depends both on its characteristic bent conformation and two conserved RNA motifs, an apical hairpin loop and a loop E. Our data suggest a general mode of subunit joining in HCV and HCV-like IRESes.
About this Structure
2HUA is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
HCV and CSFV IRES domain II mediate eIF2 release during 80S ribosome assembly., Locker N, Easton LE, Lukavsky PJ, EMBO J. 2007 Feb 7;26(3):795-805. Epub 2007 Jan 25. PMID:17255934
Page seeded by OCA on Thu Feb 21 17:45:48 2008
