2i7e
From Proteopedia
(New page: 200px<br /><applet load="2i7e" size="350" color="white" frame="true" align="right" spinBox="true" caption="2i7e" /> '''GAAA tetralooop receptor complex with associ...) |
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==Overview== | ==Overview== | ||
| - | Metal ions are critical for the proper folding of RNA, and the GAAA | + | Metal ions are critical for the proper folding of RNA, and the GAAA tetraloop-receptor is necessary for the optimal folding and function of many RNAs. We have used NMR to investigate the role of metal ions in the structure of the tetraloop-receptor in solution. The NMR data indicate native tertiary structure is formed under a wide range of ionic conditions. The lack of conformational adaptation in response to very different ionic conditions argues against a structural role for divalent ions. Nuclear Overhauser effects to cobalt hexammine and paramagnetic relaxation enhancement induced by manganese ions were used to determine the NMR structures of the tetraloop receptor in association with metal ions, providing the first atomic-level view of these interactions in the solution state. Five manganese and two cobalt hexammine ions could be localized to the RNA surface. The locations of the associated metal ions are similar, but not identical to, those of previously determined crystal structures. The sites of association are in general agreement with nonlinear Poisson-Boltzmann calculations of the electrostatic surface, emphasizing the general importance of diffusely associated ions in RNA tertiary structure. |
==About this Structure== | ==About this Structure== | ||
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Role of metal ions in the tetraloop-receptor complex as analyzed by NMR., Davis JH, Foster TR, Tonelli M, Butcher SE, RNA. 2007 Jan;13(1):76-86. Epub 2006 Nov 21. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17119098 17119098] | Role of metal ions in the tetraloop-receptor complex as analyzed by NMR., Davis JH, Foster TR, Tonelli M, Butcher SE, RNA. 2007 Jan;13(1):76-86. Epub 2006 Nov 21. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17119098 17119098] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Butcher, S | + | [[Category: Butcher, S E.]] |
| - | [[Category: Davis, J | + | [[Category: Davis, J H.]] |
[[Category: NCO]] | [[Category: NCO]] | ||
[[Category: 11-nucleotide receptor]] | [[Category: 11-nucleotide receptor]] | ||
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[[Category: rna tertiary structure]] | [[Category: rna tertiary structure]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:49:54 2008'' |
Revision as of 15:49, 21 February 2008
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GAAA tetralooop receptor complex with associated cobalt hexammine.
Overview
Metal ions are critical for the proper folding of RNA, and the GAAA tetraloop-receptor is necessary for the optimal folding and function of many RNAs. We have used NMR to investigate the role of metal ions in the structure of the tetraloop-receptor in solution. The NMR data indicate native tertiary structure is formed under a wide range of ionic conditions. The lack of conformational adaptation in response to very different ionic conditions argues against a structural role for divalent ions. Nuclear Overhauser effects to cobalt hexammine and paramagnetic relaxation enhancement induced by manganese ions were used to determine the NMR structures of the tetraloop receptor in association with metal ions, providing the first atomic-level view of these interactions in the solution state. Five manganese and two cobalt hexammine ions could be localized to the RNA surface. The locations of the associated metal ions are similar, but not identical to, those of previously determined crystal structures. The sites of association are in general agreement with nonlinear Poisson-Boltzmann calculations of the electrostatic surface, emphasizing the general importance of diffusely associated ions in RNA tertiary structure.
About this Structure
2I7E is a Protein complex structure of sequences from [1] with as ligand. Full crystallographic information is available from OCA.
Reference
Role of metal ions in the tetraloop-receptor complex as analyzed by NMR., Davis JH, Foster TR, Tonelli M, Butcher SE, RNA. 2007 Jan;13(1):76-86. Epub 2006 Nov 21. PMID:17119098
Page seeded by OCA on Thu Feb 21 17:49:54 2008
