3ako

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[[Image:3ako.jpg|left|200px]]
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==Crystal Structure of the Reassembled Venus==
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<StructureSection load='3ako' size='340' side='right' caption='[[3ako]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3ako]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Other_sequences Other sequences]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AKO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3AKO FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PE8:3,6,9,12,15,18,21-HEPTAOXATRICOSANE-1,23-DIOL'>PE8</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
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<tr><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CR2:{(4Z)-2-(AMINOMETHYL)-4-[(4-HYDROXYPHENYL)METHYLIDENE]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CR2</scene></td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ako FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ako OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ako RCSB], [http://www.ebi.ac.uk/pdbsum/3ako PDBsum]</span></td></tr>
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<table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Bimolecular fluorescence complementation (BiFC) assay has been used widely to visualize protein-protein interactions in cells. However, there is a problem that fluorescent protein fragments have an ability to associate with each other independent of an interaction between proteins fused to the fragments. To facilitate the BiFC assay, we have attempted to determine the structure and characteristics of reassembled fluorescent protein, Venus. The anion-exchange chromatography showed an oligomer and a monomer of reassembled Venus. Our results suggested that the oligomer was formed by beta-strands swapping without any serious steric clashes and was converted to the monomer. Crystal structure of reassembled Venus had an 11-stranded beta-barrel fold, typical of GFP-derived fluorescent proteins. Based on the structural features, we have mutated to beta-strand 7 and measured T(m) values. The results have revealed that the mutation influences the thermal stability of reassembled fluorescent complex.
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Structure and characteristics of reassembled fluorescent protein, a new insight into the reassembly mechanisms.,Isogai M, Kawamoto Y, Inahata K, Fukada H, Sugimoto K, Tada T Bioorg Med Chem Lett. 2011 May 15;21(10):3021-4. Epub 2011 Mar 16. PMID:21463942<ref>PMID:21463942</ref>
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The line below this paragraph, containing "STRUCTURE_3ako", creates the "Structure Box" on the page.
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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or leave the SCENE parameter empty for the default display.
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-->
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{{STRUCTURE_3ako| PDB=3ako | SCENE= }}
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===Crystal Structure of the Reassembled Venus===
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
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<references/>
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The line below this paragraph, {{ABSTRACT_PUBMED_21463942}}, adds the Publication Abstract to the page
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__TOC__
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(as it appears on PubMed at http://www.pubmed.gov), where 21463942 is the PubMed ID number.
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</StructureSection>
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{{ABSTRACT_PUBMED_21463942}}
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==About this Structure==
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[[3ako]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Other_sequences Other sequences]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AKO OCA].
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==Reference==
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<ref group="xtra">PMID:021463942</ref><references group="xtra"/>
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[[Category: Other sequences]]
[[Category: Other sequences]]
[[Category: Isogai, M.]]
[[Category: Isogai, M.]]

Revision as of 11:04, 14 May 2014

Crystal Structure of the Reassembled Venus

3ako, resolution 2.10Å

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