3skd

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:3skd.png|left|200px]]
+
==Crystal structure of the Thermus thermophilus cas3 HD domain in the presence of Ni2+==
 +
<StructureSection load='3skd' size='340' side='right' caption='[[3skd]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[3skd]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus_hb8 Thermus thermophilus hb8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SKD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3SKD FirstGlance]. <br>
 +
</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene><br>
 +
<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TTHB187 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300852 Thermus thermophilus HB8])</td></tr>
 +
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3skd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3skd OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3skd RCSB], [http://www.ebi.ac.uk/pdbsum/3skd PDBsum]</span></td></tr>
 +
<table>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
RNA transcribed from clustered regularly interspaced short palindromic repeats (CRISPRs) protects many prokaryotes from invasion by foreign DNA such as viruses, conjugative plasmids and transposable elements. CRISPR-associated protein 3 (Cas3) is essential for this CRISPR protection and is thought to mediate cleavage of the foreign DNA through its N-terminal histidine-aspartate (HD) domain. We report here the 1.8 A crystal structure of the HD domain of cas3 from Thermus thermophilus HB8. Structural and biochemical studies predict that this enzyme binds two metal ions at its active site. We also demonstrate that the single-stranded DNA endonuclease activity of this T. thermophilus domain is activated not by magnesium, but by transition metal ions such as manganese and nickel. Structure-guided mutagenesis confirms the importance of the metal binding residues for the nuclease activity, and identifies other active site residues. Overall, these results provide a framework for understanding the role of cas3 in the CRISPR system.
-
<!--
+
Structural and biochemical analysis of the nuclease domain of the clustered regularly interspaced short palindromic repeat (CRISPR) associated protein 3(CAS3).,Mulepati S, Bailey S J Biol Chem. 2011 Jul 20. PMID:21775431<ref>PMID:21775431</ref>
-
The line below this paragraph, containing "STRUCTURE_3skd", creates the "Structure Box" on the page.
+
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
-
or leave the SCENE parameter empty for the default display.
+
-
-->
+
-
{{STRUCTURE_3skd| PDB=3skd | SCENE= }}
+
-
===Crystal structure of the Thermus thermophilus cas3 HD domain in the presence of Ni2+===
+
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-
 
+
</div>
-
 
+
== References ==
-
<!--
+
<references/>
-
The line below this paragraph, {{ABSTRACT_PUBMED_21775431}}, adds the Publication Abstract to the page
+
__TOC__
-
(as it appears on PubMed at http://www.pubmed.gov), where 21775431 is the PubMed ID number.
+
</StructureSection>
-
-->
+
-
{{ABSTRACT_PUBMED_21775431}}
+
-
 
+
-
==About this Structure==
+
-
[[3skd]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus_hb8 Thermus thermophilus hb8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SKD OCA].
+
-
 
+
-
==Reference==
+
-
<ref group="xtra">PMID:021775431</ref><references group="xtra"/>
+
[[Category: Thermus thermophilus hb8]]
[[Category: Thermus thermophilus hb8]]
[[Category: Bailey, S.]]
[[Category: Bailey, S.]]

Revision as of 05:25, 5 June 2014

Crystal structure of the Thermus thermophilus cas3 HD domain in the presence of Ni2+

3skd, resolution 2.00Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3skd"
Views
Personal tools
Navigation
Toolbox