3bw3

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[[Image:3bw3.png|left|200px]]
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==Crystal structures and site-directed mutagenesis study of nitroalkane oxidase from Streptomyces ansochromogenes==
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<StructureSection load='3bw3' size='340' side='right' caption='[[3bw3]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3bw3]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_ansochromogenes Streptomyces ansochromogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BW3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3BW3 FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=NIE:NITROETHANE'>NIE</scene><br>
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<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3bw2|3bw2]], [[3bw4|3bw4]]</td></tr>
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<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">2-npdl ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=115647 Streptomyces ansochromogenes])</td></tr>
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<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Nitroalkane_oxidase Nitroalkane oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.3.1 1.7.3.1] </span></td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3bw3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bw3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3bw3 RCSB], [http://www.ebi.ac.uk/pdbsum/3bw3 PDBsum]</span></td></tr>
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<table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bw/3bw3_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Nitroalkane oxidase (NAO) catalyzes neutral nitroalkanes to their corresponding aldehydes or ketones, hydrogen peroxide and nitrite. The crystal structure of NAO from Streptomyces ansochromogenes was determined; it consists of two domains, a TIM barrel domain bound to FMN and C-terminal domain with a novel folding pattern. Site-directed mutagenesis of His179, which is spatially adjacent to FMN, resulted in the loss of enzyme activity, demonstrating that this amino acid residue is important for catalysis. The crystal structure of mutant H179D-nitroethane was also analyzed. Interestingly, Sa-NAO shows the typical function as nitroalkane oxidase but its structure is similar to that of 2-nitropropane dioxygenase. Overall, these results suggest that Sa-NAO is a novel nitroalkane oxidase with TIM barrel structure.
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Crystal structure and site-directed mutagenesis of a nitroalkane oxidase from Streptomyces ansochromogenes.,Li Y, Gao Z, Hou H, Li L, Zhang J, Yang H, Dong Y, Tan H Biochem Biophys Res Commun. 2011 Feb 18;405(3):344-8. Epub 2010 Dec 11. PMID:21147069<ref>PMID:21147069</ref>
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The line below this paragraph, containing "STRUCTURE_3bw3", creates the "Structure Box" on the page.
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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or leave the SCENE parameter empty for the default display.
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-->
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{{STRUCTURE_3bw3| PDB=3bw3 | SCENE= }}
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===Crystal structures and site-directed mutagenesis study of nitroalkane oxidase from Streptomyces ansochromogenes===
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
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<!--
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<references/>
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The line below this paragraph, {{ABSTRACT_PUBMED_21147069}}, adds the Publication Abstract to the page
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__TOC__
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(as it appears on PubMed at http://www.pubmed.gov), where 21147069 is the PubMed ID number.
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</StructureSection>
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-->
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{{ABSTRACT_PUBMED_21147069}}
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==About this Structure==
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[[3bw3]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_ansochromogenes Streptomyces ansochromogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BW3 OCA].
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==Reference==
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<ref group="xtra">PMID:021147069</ref><references group="xtra"/>
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[[Category: Nitroalkane oxidase]]
[[Category: Nitroalkane oxidase]]
[[Category: Streptomyces ansochromogenes]]
[[Category: Streptomyces ansochromogenes]]

Revision as of 09:52, 21 May 2014

Crystal structures and site-directed mutagenesis study of nitroalkane oxidase from Streptomyces ansochromogenes

3bw3, resolution 2.20Å

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