378d
From Proteopedia
(New page: 200px<br /><applet load="378d" size="350" color="white" frame="true" align="right" spinBox="true" caption="378d, resolution 2.400Å" /> '''STRUCTURE OF THE SI...) |
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==Overview== | ==Overview== | ||
| - | The 2.40 A resolution crystal structure of a side-by-side binding of | + | The 2.40 A resolution crystal structure of a side-by-side binding of distamycin A molecules to a DNA octamer d(GTATATAC)2 with an extended alternating TA sequence has been determined. The unit-cell parameters are a = 29.55, b = 42.18, c = 43.38 A, beta = 96.56 degrees, space group P21, with two molecules in the asymmetric unit, in contrast to all previous side-by-side distamycin-DNA complexes which have only a single DNA strand and one drug molecule in the asymmetric unit. The structure was solved by the molecular-replacement method and refined to an R index of 21.0% using 3467 reflections [>/= 2sigma(F)]. The minor grooves of the DNA molecules bind two side-by-side antiparallel staggered distamycins spanning about five base pairs and virtually covering the entire length of the DNA. The octamer duplexes exhibit low-high alternations in the helical twist, sugar puckering and the C-O3' and O3'-P torsion angles, similar to the earlier side-by-side complexes containing inosine bases. The molecules are stacked one over the other along the ac diagonal in an infinite pseudo-continuous helical column with no lateral interactions. |
==About this Structure== | ==About this Structure== | ||
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Structure of the side-by-side binding of distamycin to d(GTATATAC)2., Mitra SN, Wahl MC, Sundaralingam M, Acta Crystallogr D Biol Crystallogr. 1999 Mar;55(Pt 3):602-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10089456 10089456] | Structure of the side-by-side binding of distamycin to d(GTATATAC)2., Mitra SN, Wahl MC, Sundaralingam M, Acta Crystallogr D Biol Crystallogr. 1999 Mar;55(Pt 3):602-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10089456 10089456] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Mitra, S | + | [[Category: Mitra, S N.]] |
[[Category: Sundaralingam, M.]] | [[Category: Sundaralingam, M.]] | ||
| - | [[Category: Wahl, M | + | [[Category: Wahl, M C.]] |
[[Category: DMY]] | [[Category: DMY]] | ||
[[Category: NA]] | [[Category: NA]] | ||
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[[Category: right handed dna]] | [[Category: right handed dna]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:02:26 2008'' |
Revision as of 17:02, 21 February 2008
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STRUCTURE OF THE SIDE-BY-SIDE BINDING OF DISTAMYCIN TO DNA
Overview
The 2.40 A resolution crystal structure of a side-by-side binding of distamycin A molecules to a DNA octamer d(GTATATAC)2 with an extended alternating TA sequence has been determined. The unit-cell parameters are a = 29.55, b = 42.18, c = 43.38 A, beta = 96.56 degrees, space group P21, with two molecules in the asymmetric unit, in contrast to all previous side-by-side distamycin-DNA complexes which have only a single DNA strand and one drug molecule in the asymmetric unit. The structure was solved by the molecular-replacement method and refined to an R index of 21.0% using 3467 reflections [>/= 2sigma(F)]. The minor grooves of the DNA molecules bind two side-by-side antiparallel staggered distamycins spanning about five base pairs and virtually covering the entire length of the DNA. The octamer duplexes exhibit low-high alternations in the helical twist, sugar puckering and the C-O3' and O3'-P torsion angles, similar to the earlier side-by-side complexes containing inosine bases. The molecules are stacked one over the other along the ac diagonal in an infinite pseudo-continuous helical column with no lateral interactions.
About this Structure
378D is a Protein complex structure of sequences from [1] with and as ligands. Full crystallographic information is available from OCA.
Reference
Structure of the side-by-side binding of distamycin to d(GTATATAC)2., Mitra SN, Wahl MC, Sundaralingam M, Acta Crystallogr D Biol Crystallogr. 1999 Mar;55(Pt 3):602-9. PMID:10089456
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