3php
From Proteopedia
(New page: 200px<br /><applet load="3php" size="350" color="white" frame="true" align="right" spinBox="true" caption="3php" /> '''STRUCTURE OF THE 3' HAIRPIN OF THE TYMV PSEU...) |
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==Overview== | ==Overview== | ||
| - | The solution structure of an RNA-hairpin present in the pseudoknot, which | + | The solution structure of an RNA-hairpin present in the pseudoknot, which is found at the 3'-terminus of turnip yellow mosaic virus genomic RNA, has been solved by nuclear magnetic resonance spectroscopy. The loop, which contains the sequence 5'-GGGUCA-3', was found to be highly structured and, contrary to expectations, does not attain its stability through GA or GC base pair formation but by triple interactions between the tilted adenosine and the minor groove sides of the first two guanosines. Interestingly, a very similar conformation was found for the cognate pseudoknot, implying that the 3'-hairpin is preformed for folding into a pseudoknotted structure. These findings suggest a mechanism of 'predetermined-fit' as a principle in RNA folding. |
==About this Structure== | ==About this Structure== | ||
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Structure of the 3'-hairpin of the TYMV pseudoknot: preformation in RNA folding., Kolk MH, van der Graaf M, Fransen CT, Wijmenga SS, Pleij CW, Heus HA, Hilbers CW, EMBO J. 1998 Dec 15;17(24):7498-504. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9857204 9857204] | Structure of the 3'-hairpin of the TYMV pseudoknot: preformation in RNA folding., Kolk MH, van der Graaf M, Fransen CT, Wijmenga SS, Pleij CW, Heus HA, Hilbers CW, EMBO J. 1998 Dec 15;17(24):7498-504. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9857204 9857204] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Graaf, M | + | [[Category: Graaf, M Van Der.]] |
| - | [[Category: Heus, H | + | [[Category: Heus, H A.]] |
| - | [[Category: Hilbers, C | + | [[Category: Hilbers, C W.]] |
| - | [[Category: Kolk, M | + | [[Category: Kolk, M H.]] |
| - | [[Category: Pleij, C | + | [[Category: Pleij, C W.A.]] |
| - | [[Category: Wijmenga, S | + | [[Category: Wijmenga, S S.]] |
[[Category: pseudoknot]] | [[Category: pseudoknot]] | ||
[[Category: ribonucleic acid]] | [[Category: ribonucleic acid]] | ||
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[[Category: tymv]] | [[Category: tymv]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:10:52 2008'' |
Revision as of 17:10, 21 February 2008
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STRUCTURE OF THE 3' HAIRPIN OF THE TYMV PSEUDOKNOT: PREFORMATION IN RNA FOLDING
Overview
The solution structure of an RNA-hairpin present in the pseudoknot, which is found at the 3'-terminus of turnip yellow mosaic virus genomic RNA, has been solved by nuclear magnetic resonance spectroscopy. The loop, which contains the sequence 5'-GGGUCA-3', was found to be highly structured and, contrary to expectations, does not attain its stability through GA or GC base pair formation but by triple interactions between the tilted adenosine and the minor groove sides of the first two guanosines. Interestingly, a very similar conformation was found for the cognate pseudoknot, implying that the 3'-hairpin is preformed for folding into a pseudoknotted structure. These findings suggest a mechanism of 'predetermined-fit' as a principle in RNA folding.
About this Structure
3PHP is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Structure of the 3'-hairpin of the TYMV pseudoknot: preformation in RNA folding., Kolk MH, van der Graaf M, Fransen CT, Wijmenga SS, Pleij CW, Heus HA, Hilbers CW, EMBO J. 1998 Dec 15;17(24):7498-504. PMID:9857204
Page seeded by OCA on Thu Feb 21 19:10:52 2008
