2v6u

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
m (Protected "2v6u" [edit=sysop:move=sysop])
Line 1: Line 1:
-
[[Image:2v6u.png|left|200px]]
+
==HIGH RESOLUTION CRYSTAL STRUCTURE OF PTERIN-4A-CARBINOLAMINE DEHYDRATASE FROM TOXOPLASMA GONDII==
 +
<StructureSection load='2v6u' size='340' side='right' caption='[[2v6u]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[2v6u]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Toxoplasma_gondii Toxoplasma gondii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V6U OCA]. <br>
 +
</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene><br>
 +
<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2v6s|2v6s]], [[2v6t|2v6t]]</td></tr>
 +
<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr>
 +
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2v6u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v6u OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2v6u RCSB], [http://www.ebi.ac.uk/pdbsum/2v6u PDBsum]</span></td></tr>
 +
<table>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/v6/2v6u_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
The enzyme pterin-4a-carbinolamine dehydratase (PCD) is important for the recycling of pterins within eukaryotic cells. A recombinant expression system for PCD from the apicomplexan parasite Toxoplasma gondii has been prepared, the protein purified and crystallised. Single crystal X-ray diffraction methods have produced a high-resolution structure (1.6A) of the apo-enzyme and a low-resolution structure (3.1A) of a complex with a substrate-like ligand dihydrobiopterin (BH(2)). Analysis of the hydrogen bonding interactions that contribute to binding BH(2) suggest that the ligand is present in an enol tautomeric state, which makes it more similar to the physiological substrate. The enzyme can process (R)- and (S)-forms of pterin-4a-carbinolamine and the ligand complex suggests that His61 and His79 are placed to act independently as general bases for catalysis of the individual enantiomers. Comparisons with orthologues from other protozoan parasites (Plasmodium falciparum and Leishmania major) and with rat PCD, for which the structure is known, indicate a high degree of sequence and structure conservation of this enzyme. The molecular determinants of ligand recognition and PCD reactivity are therefore highly conserved across species.
-
<!--
+
Crystal structures of Toxoplasma gondii pterin-4a-carbinolamine dehydratase and comparisons with mammalian and parasite orthologues.,Cameron S, Fyffe SA, Goldie S, Hunter WN Mol Biochem Parasitol. 2008 Apr;158(2):131-8. Epub 2007 Dec 15. PMID:18215430<ref>PMID:18215430</ref>
-
The line below this paragraph, containing "STRUCTURE_2v6u", creates the "Structure Box" on the page.
+
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
-
or leave the SCENE parameter empty for the default display.
+
-
-->
+
-
{{STRUCTURE_2v6u| PDB=2v6u | SCENE= }}
+
-
===HIGH RESOLUTION CRYSTAL STRUCTURE OF PTERIN-4A-CARBINOLAMINE DEHYDRATASE FROM TOXOPLASMA GONDII===
+
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-
 
+
</div>
-
 
+
== References ==
-
<!--
+
<references/>
-
The line below this paragraph, {{ABSTRACT_PUBMED_18215430}}, adds the Publication Abstract to the page
+
__TOC__
-
(as it appears on PubMed at http://www.pubmed.gov), where 18215430 is the PubMed ID number.
+
</StructureSection>
-
-->
+
-
{{ABSTRACT_PUBMED_18215430}}
+
-
 
+
-
==About this Structure==
+
-
[[2v6u]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Toxoplasma_gondii Toxoplasma gondii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V6U OCA].
+
-
 
+
-
==Reference==
+
-
<ref group="xtra">PMID:018215430</ref><references group="xtra"/>
+
[[Category: 4a-hydroxytetrahydrobiopterin dehydratase]]
[[Category: 4a-hydroxytetrahydrobiopterin dehydratase]]
[[Category: Toxoplasma gondii]]
[[Category: Toxoplasma gondii]]

Revision as of 08:32, 7 May 2014

HIGH RESOLUTION CRYSTAL STRUCTURE OF PTERIN-4A-CARBINOLAMINE DEHYDRATASE FROM TOXOPLASMA GONDII

2v6u, resolution 1.60Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2v6u"
Views
Personal tools
Navigation
Toolbox