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2iu5

From Proteopedia

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[[Image:2iu5.png|left|200px]]
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==DIHYDROXYACETONE KINASE OPERON ACTIVATOR DHAS==
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<StructureSection load='2iu5' size='340' side='right' caption='[[2iu5]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
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== Structural highlights ==
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[[2iu5]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lactococcus_lactis_subsp._lactis_il1403 Lactococcus lactis subsp. lactis il1403]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IU5 OCA]. <br>
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<b>Activity:</b> <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span><br>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/iu/2iu5_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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== Publication Abstract from PubMed ==
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Dihydroxyacetone (Dha) kinases are a novel family of kinases with signaling and metabolic functions. Here we report the x-ray structures of the transcriptional activator DhaS and the coactivator DhaQ and characterize their function. DhaQ is a paralog of the Dha binding Dha kinase subunit; DhaS belongs to the family of TetR repressors although, unlike all known members of this family, it is a transcriptional activator. DhaQ and DhaS form a stable complex that in the presence of Dha activates transcription of the Lactococcus lactis dha operon. Dha covalently binds to DhaQ through a hemiaminal bond with a histidine and thereby induces a conformational change, which is propagated to the surface via a cantilever-like structure. DhaS binding protects an inverted repeat whose sequence is GGACACATN6ATTTGTCC and renders two GC base pairs of the operator DNA hypersensitive to DNase I cleavage. The proximal half-site of the inverted repeat partially overlaps with the predicted -35 consensus sequence of the dha promoter.
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Regulation of the Dha operon of Lactococcus lactis: a deviation from the rule followed by the Tetr family of transcription regulators.,Christen S, Srinivas A, Bahler P, Zeller A, Pridmore D, Bieniossek C, Baumann U, Erni B J Biol Chem. 2006 Aug 11;281(32):23129-37. Epub 2006 Jun 7. PMID:16760471<ref>PMID:16760471</ref>
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The line below this paragraph, containing "STRUCTURE_2iu5", creates the "Structure Box" on the page.
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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or leave the SCENE parameter empty for the default display.
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-->
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{{STRUCTURE_2iu5| PDB=2iu5 | SCENE= }}
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===DIHYDROXYACETONE KINASE OPERON ACTIVATOR DHAS===
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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== References ==
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<references/>
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<!--
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__TOC__
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The line below this paragraph, {{ABSTRACT_PUBMED_16760471}}, adds the Publication Abstract to the page
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</StructureSection>
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(as it appears on PubMed at http://www.pubmed.gov), where 16760471 is the PubMed ID number.
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-->
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{{ABSTRACT_PUBMED_16760471}}
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==About this Structure==
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[[2iu5]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lactococcus_lactis_subsp._lactis_il1403 Lactococcus lactis subsp. lactis il1403]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IU5 OCA].
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==Reference==
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<ref group="xtra">PMID:016760471</ref><references group="xtra"/>
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[[Category: Lactococcus lactis subsp. lactis il1403]]
[[Category: Lactococcus lactis subsp. lactis il1403]]
[[Category: Baumann, U.]]
[[Category: Baumann, U.]]

Revision as of 08:27, 30 April 2014

DIHYDROXYACETONE KINASE OPERON ACTIVATOR DHAS

2iu5, resolution 1.60Å

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