454d
From Proteopedia
(New page: 200px<br /><applet load="454d" size="350" color="white" frame="true" align="right" spinBox="true" caption="454d, resolution 1.2Å" /> '''INTERCALATION AND MAJ...) |
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==Overview== | ==Overview== | ||
| - | Intercalating complexes of rhodium(III) are strong photo-oxidants that | + | Intercalating complexes of rhodium(III) are strong photo-oxidants that promote DNA strand cleavage or electron transfer through the double helix. The 1.2 A resolution crystal structure of a sequence-specific rhodium intercalator bound to a DNA helix provides a rationale for the sequence specificity of rhodium intercalators. It also explains how intercalation in the center of an oligonucleotide modifies DNA conformation. The rhodium complex intercalates via the major groove where specific contacts are formed with the edges of the bases at the target site. The phi ligand is deeply inserted into the DNA base pair stack. The primary conformational change of the DNA is a doubling of the rise per residue, with no change in sugar pucker from B-form DNA. Based upon the five crystallographically independent views of an intercalated DNA helix observed in this structure, the intercalator may be considered as an additional base pair with specific functional groups positioned in the major groove. |
==About this Structure== | ==About this Structure== | ||
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Structure of a photoactive rhodium complex intercalated into DNA., Kielkopf CL, Erkkila KE, Hudson BP, Barton JK, Rees DC, Nat Struct Biol. 2000 Feb;7(2):117-21. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10655613 10655613] | Structure of a photoactive rhodium complex intercalated into DNA., Kielkopf CL, Erkkila KE, Hudson BP, Barton JK, Rees DC, Nat Struct Biol. 2000 Feb;7(2):117-21. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10655613 10655613] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Barton, J | + | [[Category: Barton, J K.]] |
| - | [[Category: Erkkila, K | + | [[Category: Erkkila, K E.]] |
| - | [[Category: Hudson, B | + | [[Category: Hudson, B P.]] |
| - | [[Category: Kielkopf, C | + | [[Category: Kielkopf, C L.]] |
| - | [[Category: Rees, D | + | [[Category: Rees, D C.]] |
[[Category: RHM]] | [[Category: RHM]] | ||
[[Category: intercalation]] | [[Category: intercalation]] | ||
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[[Category: photoexcitable]] | [[Category: photoexcitable]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:12:23 2008'' |
Revision as of 17:12, 21 February 2008
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INTERCALATION AND MAJOR GROOVE RECOGNITION IN THE 1.2 A RESOLUTION CRYSTAL STRUCTURE OF RH[ME2TRIEN]PHI BOUND TO 5'-G(5IU)TGCAAC-3'
Overview
Intercalating complexes of rhodium(III) are strong photo-oxidants that promote DNA strand cleavage or electron transfer through the double helix. The 1.2 A resolution crystal structure of a sequence-specific rhodium intercalator bound to a DNA helix provides a rationale for the sequence specificity of rhodium intercalators. It also explains how intercalation in the center of an oligonucleotide modifies DNA conformation. The rhodium complex intercalates via the major groove where specific contacts are formed with the edges of the bases at the target site. The phi ligand is deeply inserted into the DNA base pair stack. The primary conformational change of the DNA is a doubling of the rise per residue, with no change in sugar pucker from B-form DNA. Based upon the five crystallographically independent views of an intercalated DNA helix observed in this structure, the intercalator may be considered as an additional base pair with specific functional groups positioned in the major groove.
About this Structure
454D is a Protein complex structure of sequences from [1] with as ligand. Full crystallographic information is available from OCA.
Reference
Structure of a photoactive rhodium complex intercalated into DNA., Kielkopf CL, Erkkila KE, Hudson BP, Barton JK, Rees DC, Nat Struct Biol. 2000 Feb;7(2):117-21. PMID:10655613
Page seeded by OCA on Thu Feb 21 19:12:23 2008
