2pbi
From Proteopedia
(Difference between revisions)
(New page: 200px<br /><applet load="2pbi" size="350" color="white" frame="true" align="right" spinBox="true" caption="2pbi, resolution 1.950Å" /> '''The multifunctional...) |
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==Overview== | ==Overview== | ||
| - | Regulators of G-protein signaling (RGS) proteins enhance the intrinsic | + | Regulators of G-protein signaling (RGS) proteins enhance the intrinsic GTPase activity of G protein alpha (Galpha) subunits and are vital for proper signaling kinetics downstream of G protein-coupled receptors (GPCRs). R7 subfamily RGS proteins specifically and obligately dimerize with the atypical G protein beta5 (Gbeta5) subunit through an internal G protein gamma (Ggamma)-subunit-like (GGL) domain. Here we present the 1.95-A crystal structure of the Gbeta5-RGS9 complex, which is essential for normal visual and neuronal signal transduction. This structure reveals a canonical RGS domain that is functionally integrated within a molecular complex that is poised for integration of multiple steps during G-protein activation and deactivation. |
==About this Structure== | ==About this Structure== | ||
| - | 2PBI is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Sites: <scene name='pdbsite=AC1:Gol Binding Site For Residue D 901'>AC1</scene>, <scene name='pdbsite=AC2:Gol Binding Site For Residue B 902'>AC2</scene>, <scene name='pdbsite=AC3:Gol Binding Site For Residue D 903'>AC3</scene>, <scene name='pdbsite=AC4:Gol Binding Site For Residue A 904'>AC4</scene>, <scene name='pdbsite=AC5:Gol Binding Site For Residue A 905'>AC5</scene>, <scene name='pdbsite=AC6:Gol Binding Site For Residue A 906'>AC6</scene>, <scene name='pdbsite=AC7:Gol Binding Site For Residue C 907'>AC7</scene>, <scene name='pdbsite=AC8:Gol Binding Site For Residue D 908'>AC8</scene> and <scene name='pdbsite=AC9:Gol Binding Site For Residue B 909'>AC9</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PBI OCA]. | + | 2PBI is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Sites: <scene name='pdbsite=AC1:Gol+Binding+Site+For+Residue+D+901'>AC1</scene>, <scene name='pdbsite=AC2:Gol+Binding+Site+For+Residue+B+902'>AC2</scene>, <scene name='pdbsite=AC3:Gol+Binding+Site+For+Residue+D+903'>AC3</scene>, <scene name='pdbsite=AC4:Gol+Binding+Site+For+Residue+A+904'>AC4</scene>, <scene name='pdbsite=AC5:Gol+Binding+Site+For+Residue+A+905'>AC5</scene>, <scene name='pdbsite=AC6:Gol+Binding+Site+For+Residue+A+906'>AC6</scene>, <scene name='pdbsite=AC7:Gol+Binding+Site+For+Residue+C+907'>AC7</scene>, <scene name='pdbsite=AC8:Gol+Binding+Site+For+Residue+D+908'>AC8</scene> and <scene name='pdbsite=AC9:Gol+Binding+Site+For+Residue+B+909'>AC9</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PBI OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of the multifunctional Gbeta5-RGS9 complex., Cheever ML, Snyder JT, Gershburg S, Siderovski DP, Harden TK, Sondek J, Nat Struct Mol Biol. 2008 Jan 20 | + | Crystal structure of the multifunctional Gbeta5-RGS9 complex., Cheever ML, Snyder JT, Gershburg S, Siderovski DP, Harden TK, Sondek J, Nat Struct Mol Biol. 2008 Feb;15(2):155-62. Epub 2008 Jan 20. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18204463 18204463] |
[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Cheever, M | + | [[Category: Cheever, M L.]] |
[[Category: Gershburg, S.]] | [[Category: Gershburg, S.]] | ||
| - | [[Category: Harden, T | + | [[Category: Harden, T K.]] |
| - | [[Category: Siderovski, D | + | [[Category: Siderovski, D P.]] |
| - | [[Category: Snyder, J | + | [[Category: Snyder, J T.]] |
[[Category: Sondek, J.]] | [[Category: Sondek, J.]] | ||
[[Category: GOL]] | [[Category: GOL]] | ||
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[[Category: signaling protein]] | [[Category: signaling protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:27:54 2008'' |
Revision as of 16:27, 21 February 2008
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The multifunctional nature of Gbeta5/RGS9 revealed from its crystal structure
Overview
Regulators of G-protein signaling (RGS) proteins enhance the intrinsic GTPase activity of G protein alpha (Galpha) subunits and are vital for proper signaling kinetics downstream of G protein-coupled receptors (GPCRs). R7 subfamily RGS proteins specifically and obligately dimerize with the atypical G protein beta5 (Gbeta5) subunit through an internal G protein gamma (Ggamma)-subunit-like (GGL) domain. Here we present the 1.95-A crystal structure of the Gbeta5-RGS9 complex, which is essential for normal visual and neuronal signal transduction. This structure reveals a canonical RGS domain that is functionally integrated within a molecular complex that is poised for integration of multiple steps during G-protein activation and deactivation.
About this Structure
2PBI is a Protein complex structure of sequences from Mus musculus with as ligand. Known structural/functional Sites: , , , , , , , and . Full crystallographic information is available from OCA.
Reference
Crystal structure of the multifunctional Gbeta5-RGS9 complex., Cheever ML, Snyder JT, Gershburg S, Siderovski DP, Harden TK, Sondek J, Nat Struct Mol Biol. 2008 Feb;15(2):155-62. Epub 2008 Jan 20. PMID:18204463
Page seeded by OCA on Thu Feb 21 18:27:54 2008
