2qyf
From Proteopedia
(New page: 200px<br /><applet load="2qyf" size="350" color="white" frame="true" align="right" spinBox="true" caption="2qyf, resolution 2.30Å" /> '''Crystal structure of...) |
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==Overview== | ==Overview== | ||
| - | The status of spindle checkpoint signaling depends on the balance of two | + | The status of spindle checkpoint signaling depends on the balance of two opposing dynamic processes that regulate the highly unusual two-state behavior of Mad2. In mitosis, a Mad1-Mad2 core complex recruits cytosolic Mad2 to kinetochores through Mad2 dimerization and converts Mad2 to a conformer amenable to Cdc20 binding, thereby facilitating checkpoint activation. p31(comet) inactivates the checkpoint through binding to Mad1- or Cdc20-bound Mad2, thereby preventing Mad2 activation and promoting the dissociation of the Mad2-Cdc20 complex. Here, we report the crystal structure of the Mad2-p31(comet) complex. The C-terminal region of Mad2 that undergoes rearrangement in different Mad2 conformers is a major structural determinant for p31(comet) binding, explaining the specificity of p31(comet) toward Mad1- or Cdc20-bound Mad2. p31(comet) adopts a fold strikingly similar to that of Mad2 and binds at the dimerization interface of Mad2. Thus, p31(comet) exploits the two-state behavior of Mad2 to block its activation by acting as an "anti-Mad2." |
==About this Structure== | ==About this Structure== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Luo, X.]] | [[Category: Luo, X.]] | ||
| - | [[Category: Tomchick, D | + | [[Category: Tomchick, D R.]] |
[[Category: cell cycle]] | [[Category: cell cycle]] | ||
[[Category: cell division]] | [[Category: cell division]] | ||
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[[Category: spindle assembly checkpoint]] | [[Category: spindle assembly checkpoint]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:43:19 2008'' |
Revision as of 16:43, 21 February 2008
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Crystal structure of the Mad2/p31(comet)/Mad2-binding peptide ternary complex
Overview
The status of spindle checkpoint signaling depends on the balance of two opposing dynamic processes that regulate the highly unusual two-state behavior of Mad2. In mitosis, a Mad1-Mad2 core complex recruits cytosolic Mad2 to kinetochores through Mad2 dimerization and converts Mad2 to a conformer amenable to Cdc20 binding, thereby facilitating checkpoint activation. p31(comet) inactivates the checkpoint through binding to Mad1- or Cdc20-bound Mad2, thereby preventing Mad2 activation and promoting the dissociation of the Mad2-Cdc20 complex. Here, we report the crystal structure of the Mad2-p31(comet) complex. The C-terminal region of Mad2 that undergoes rearrangement in different Mad2 conformers is a major structural determinant for p31(comet) binding, explaining the specificity of p31(comet) toward Mad1- or Cdc20-bound Mad2. p31(comet) adopts a fold strikingly similar to that of Mad2 and binds at the dimerization interface of Mad2. Thus, p31(comet) exploits the two-state behavior of Mad2 to block its activation by acting as an "anti-Mad2."
About this Structure
2QYF is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
p31comet blocks Mad2 activation through structural mimicry., Yang M, Li B, Tomchick DR, Machius M, Rizo J, Yu H, Luo X, Cell. 2007 Nov 16;131(4):744-55. PMID:18022368
Page seeded by OCA on Thu Feb 21 18:43:19 2008
