1a9l
From Proteopedia
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==Overview== | ==Overview== | ||
| - | The structure of the bulge-helix-bulge motif that constitutes the | + | The structure of the bulge-helix-bulge motif that constitutes the intron/exon splice site in H. volcanii pre-tRNATrp has been determined by NMR spectroscopy. The conformations of the two 3 nt bulges, where the pre-tRNA is cleaved, are stabilized by stacking interactions between bulge nucleotides and bases in the adjacent Watson-Crick helices and by a network of backbone hydrogen bonds. Both bulges are presented on the same minor groove face of the central 4 bp helix, and the overall structure has approximate two-fold symmetry, which makes it well-suited for attack by archaeal splicing endonucleases, which are symmetric dimers. |
==About this Structure== | ==About this Structure== | ||
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Solution structure of a substrate for the archaeal pre-tRNA splicing endonucleases: the bulge-helix-bulge motif., Diener JL, Moore PB, Mol Cell. 1998 May;1(6):883-94. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9660971 9660971] | Solution structure of a substrate for the archaeal pre-tRNA splicing endonucleases: the bulge-helix-bulge motif., Diener JL, Moore PB, Mol Cell. 1998 May;1(6):883-94. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9660971 9660971] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Diener, J | + | [[Category: Diener, J L.]] |
| - | [[Category: Moore, P | + | [[Category: Moore, P B.]] |
[[Category: archaea]] | [[Category: archaea]] | ||
[[Category: archaeal bulge-helix-bulge motif]] | [[Category: archaeal bulge-helix-bulge motif]] | ||
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[[Category: rna structure]] | [[Category: rna structure]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:42:24 2008'' |
Revision as of 09:42, 21 February 2008
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SOLUTION STRUCTURE OF A SUBSTRATE FOR THE ARCHAEAL PRE-TRNA SPLICING ENDONUCLEASES: THE BULGE-HELIX-BULGE MOTIF, NMR, 12 STRUCTURES
Overview
The structure of the bulge-helix-bulge motif that constitutes the intron/exon splice site in H. volcanii pre-tRNATrp has been determined by NMR spectroscopy. The conformations of the two 3 nt bulges, where the pre-tRNA is cleaved, are stabilized by stacking interactions between bulge nucleotides and bases in the adjacent Watson-Crick helices and by a network of backbone hydrogen bonds. Both bulges are presented on the same minor groove face of the central 4 bp helix, and the overall structure has approximate two-fold symmetry, which makes it well-suited for attack by archaeal splicing endonucleases, which are symmetric dimers.
About this Structure
1A9L is a Protein complex structure of sequences from [1]. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Solution structure of a substrate for the archaeal pre-tRNA splicing endonucleases: the bulge-helix-bulge motif., Diener JL, Moore PB, Mol Cell. 1998 May;1(6):883-94. PMID:9660971
Page seeded by OCA on Thu Feb 21 11:42:24 2008
