1aa6
From Proteopedia
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==Overview== | ==Overview== | ||
| - | Formate dehydrogenase H from Escherichia coli contains selenocysteine | + | Formate dehydrogenase H from Escherichia coli contains selenocysteine (SeCys), molybdenum, two molybdopterin guanine dinucleotide (MGD) cofactors, and an Fe4S4 cluster at the active site and catalyzes the two-electron oxidation of formate to carbon dioxide. The crystal structures of the oxidized [Mo(VI), Fe4S4(ox)] form of formate dehydrogenase H (with and without bound inhibitor) and the reduced [Mo(IV), Fe4S4(red)] form have been determined, revealing a four-domain alphabeta structure with the molybdenum directly coordinated to selenium and both MGD cofactors. These structures suggest a reaction mechanism that directly involves SeCys140 and His141 in proton abstraction and the molybdenum, molybdopterin, Lys44, and the Fe4S4 cluster in electron transfer. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Formate dehydrogenase]] | [[Category: Formate dehydrogenase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Boyington, J | + | [[Category: Boyington, J C.]] |
| - | [[Category: Sun, P | + | [[Category: Sun, P D.]] |
[[Category: 4MO]] | [[Category: 4MO]] | ||
[[Category: MGD]] | [[Category: MGD]] | ||
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[[Category: selenocysteine]] | [[Category: selenocysteine]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:42:33 2008'' |
Revision as of 09:42, 21 February 2008
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REDUCED FORM OF FORMATE DEHYDROGENASE H FROM E. COLI
Overview
Formate dehydrogenase H from Escherichia coli contains selenocysteine (SeCys), molybdenum, two molybdopterin guanine dinucleotide (MGD) cofactors, and an Fe4S4 cluster at the active site and catalyzes the two-electron oxidation of formate to carbon dioxide. The crystal structures of the oxidized [Mo(VI), Fe4S4(ox)] form of formate dehydrogenase H (with and without bound inhibitor) and the reduced [Mo(IV), Fe4S4(red)] form have been determined, revealing a four-domain alphabeta structure with the molybdenum directly coordinated to selenium and both MGD cofactors. These structures suggest a reaction mechanism that directly involves SeCys140 and His141 in proton abstraction and the molybdenum, molybdopterin, Lys44, and the Fe4S4 cluster in electron transfer.
About this Structure
1AA6 is a Single protein structure of sequence from Escherichia coli with , and as ligands. Active as Formate dehydrogenase, with EC number 1.2.1.2 Known structural/functional Sites: and . Full crystallographic information is available from OCA.
Reference
Crystal structure of formate dehydrogenase H: catalysis involving Mo, molybdopterin, selenocysteine, and an Fe4S4 cluster., Boyington JC, Gladyshev VN, Khangulov SV, Stadtman TC, Sun PD, Science. 1997 Feb 28;275(5304):1305-8. PMID:9036855
Page seeded by OCA on Thu Feb 21 11:42:33 2008
Categories: Escherichia coli | Formate dehydrogenase | Single protein | Boyington, J C. | Sun, P D. | 4MO | MGD | SF4 | Anaerobic | Dehydrogenase | Fe4s4 | Formate | Iron sulfur cluster | Mgd | Molybdenum | Molybdopterin | Molybdopterin guanine dinucleotide | Mpt | Oxidoreductase | Secys | Selenium | Selenocysteine
