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1ac5
From Proteopedia
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Revision as of 12:42, 30 October 2007
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CRYSTAL STRUCTURE OF KEX1(DELTA)P, A PROHORMONE-PROCESSING CARBOXYPEPTIDASE FROM SACCHAROMYCES CEREVISIAE
Overview
A soluble form of the killer factor and prohormone-processing, carboxypeptidase, "Kex1 delta p," from Saccharomyces cerevisiae, has been, crystallized in 17-22% poly(enthylene glycol) methyl ether (average M(r) =, 5,000), 100 mM ammonium acetate, 5% glycerol, pH 6.5, at 20 degrees C. A, native data set (2.8 A resolution) and four derivative data sets (3.0-3.2, A resolution) were collected at the Photon Factory (lambda = 1.0 A). The, crystals belong to space group P2(1)2(1)2(1) with a =56.6 A, b = 84.0 A, c, = 111.8 A. Freezing a Kex1 delta p crystal has facilitated the collection, of a 2.4-A data set using a rotating anode source (lambda = 1.5418 A)., Molecular replacement models have been built based on the structures of, wheat serine carboxypeptidase (CPDW-II; Liao DI et al., 1992, ... [(full description)]
About this Structure
1AC5 is a [Single protein] structure of sequence from [Saccharomyces cerevisiae] with NAG as [ligand]. Active as [Carboxypeptidase D], with EC number [3.4.16.6]. Structure known Active Sites: 176, 383 and 448. Full crystallographic information is available from [OCA].
Reference
Crystallization of a soluble form of the Kex1p serine carboxypeptidase from Saccharomyces cerevisiae., Shilton BH, Li Y, Tessier D, Thomas DY, Cygler M, Protein Sci. 1996 Feb;5(2):395-7. PMID:8745419
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