1ckn
From Proteopedia
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==Overview== | ==Overview== | ||
| - | We have solved the crystal structure of an mRNA capping enzyme at 2.5 A | + | We have solved the crystal structure of an mRNA capping enzyme at 2.5 A resolution. The enzyme comprises two domains with a deep, but narrow, cleft between them. The two molecules in the crystallographic asymmetric unit adopt very different conformations; both contain a bound GTP, but one protein molecule is in an open conformation while the other is in a closed conformation. Only in the closed conformation is the enzyme able to bind manganese ions and undergo catalysis within the crystals to yield the covalent guanylated enzyme intermediate. These structures provide direct evidence for a mechanism that involves a significant conformational change in the enzyme during catalysis. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: mRNA guanylyltransferase]] | [[Category: mRNA guanylyltransferase]] | ||
| - | [[Category: Doherty, A | + | [[Category: Doherty, A J.]] |
[[Category: Hakansson, K.]] | [[Category: Hakansson, K.]] | ||
| - | [[Category: Wigley, D | + | [[Category: Wigley, D B.]] |
[[Category: GTP]] | [[Category: GTP]] | ||
[[Category: MN]] | [[Category: MN]] | ||
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[[Category: nucleotidyltransferase]] | [[Category: nucleotidyltransferase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:07:00 2008'' |
Revision as of 10:07, 21 February 2008
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STRUCTURE OF GUANYLYLATED MRNA CAPPING ENZYME COMPLEXED WITH GTP
Overview
We have solved the crystal structure of an mRNA capping enzyme at 2.5 A resolution. The enzyme comprises two domains with a deep, but narrow, cleft between them. The two molecules in the crystallographic asymmetric unit adopt very different conformations; both contain a bound GTP, but one protein molecule is in an open conformation while the other is in a closed conformation. Only in the closed conformation is the enzyme able to bind manganese ions and undergo catalysis within the crystals to yield the covalent guanylated enzyme intermediate. These structures provide direct evidence for a mechanism that involves a significant conformational change in the enzyme during catalysis.
About this Structure
1CKN is a Single protein structure of sequence from Paramecium bursaria chlorella virus 1 with , and as ligands. Active as mRNA guanylyltransferase, with EC number 2.7.7.50 Known structural/functional Sites: and . Full crystallographic information is available from OCA.
Reference
X-ray crystallography reveals a large conformational change during guanyl transfer by mRNA capping enzymes., Hakansson K, Doherty AJ, Shuman S, Wigley DB, Cell. 1997 May 16;89(4):545-53. PMID:9160746
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