1dyt
From Proteopedia
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==Overview== | ==Overview== | ||
| - | Eosinophil cationic protein (ECP; RNase 3) is a human ribonuclease found | + | Eosinophil cationic protein (ECP; RNase 3) is a human ribonuclease found only in eosinophil leukocytes that belongs to the RNase A superfamily. This enzyme is bactericidal, helminthotoxic and cytotoxic to mammalian cells and tissues. The protein has been cloned, heterologously overexpressed, purified and crystallized. Its crystal structure has been determined and refined using data up to 1. 75 A resolution. The molecule displays the alpha+beta folding topology typical for members of the ribonuclease A superfamily. The catalytic active site residues are conserved with respect to other ribonucleases of the superfamily but some differences appear at substrate recognition subsites, which may account, in part, for the low catalytic activity. Most strikingly, 19 surface-located arginine residues confer a strong basic character to the protein. The high concentration of positive charges and the particular orientation of the side-chains of these residues may also be related to the low activity of ECP as a ribonuclease and provides an explanation for its unique cytotoxic role through cell membrane disruption. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Coll, M.]] | [[Category: Coll, M.]] | ||
| - | [[Category: Gomis-Rueth, F | + | [[Category: Gomis-Rueth, F X.]] |
| - | [[Category: Llorens, R | + | [[Category: Llorens, R De.]] |
[[Category: Maeda, T.]] | [[Category: Maeda, T.]] | ||
[[Category: Mallorqui-Fernandez, G.]] | [[Category: Mallorqui-Fernandez, G.]] | ||
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[[Category: rnase 3]] | [[Category: rnase 3]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:21:58 2008'' |
Revision as of 10:21, 21 February 2008
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X-RAY CRYSTAL STRUCTURE OF ECP (RNASE 3) AT 1.75 A
Overview
Eosinophil cationic protein (ECP; RNase 3) is a human ribonuclease found only in eosinophil leukocytes that belongs to the RNase A superfamily. This enzyme is bactericidal, helminthotoxic and cytotoxic to mammalian cells and tissues. The protein has been cloned, heterologously overexpressed, purified and crystallized. Its crystal structure has been determined and refined using data up to 1. 75 A resolution. The molecule displays the alpha+beta folding topology typical for members of the ribonuclease A superfamily. The catalytic active site residues are conserved with respect to other ribonucleases of the superfamily but some differences appear at substrate recognition subsites, which may account, in part, for the low catalytic activity. Most strikingly, 19 surface-located arginine residues confer a strong basic character to the protein. The high concentration of positive charges and the particular orientation of the side-chains of these residues may also be related to the low activity of ECP as a ribonuclease and provides an explanation for its unique cytotoxic role through cell membrane disruption.
About this Structure
1DYT is a Single protein structure of sequence from Homo sapiens with and as ligands. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Three-dimensional crystal structure of human eosinophil cationic protein (RNase 3) at 1.75 A resolution., Mallorqui-Fernandez G, Pous J, Peracaula R, Aymami J, Maeda T, Tada H, Yamada H, Seno M, de Llorens R, Gomis-Ruth FX, Coll M, J Mol Biol. 2000 Jul 28;300(5):1297-307. PMID:10903870
Page seeded by OCA on Thu Feb 21 12:21:58 2008
Categories: Homo sapiens | Single protein | Coll, M. | Gomis-Rueth, F X. | Llorens, R De. | Maeda, T. | Mallorqui-Fernandez, G. | Peracaula, R. | Pous, J. | Seno, M. | Tada, H. | Yamada, H. | CIT | FE | Ecp | Eosinophil cationic protein | Rnase 3
