1gvz
From Proteopedia
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==Overview== | ==Overview== | ||
| - | Prostate-specific kallikrein, a member of the gene family of serine | + | Prostate-specific kallikrein, a member of the gene family of serine proteases, was initially discovered in semen and is the most useful serum marker for prostate cancer diagnosis and prognosis. We report the crystal structure at 1.42A resolution of horse prostate kallikrein (HPK). This is the first structure of a serine protease purified from seminal plasma. HPK shares extensive sequence homology with human prostate-specific antigen (PSA), including a predicted chymotrypsin-like specificity, as suggested by the presence of a serine residue at position S1 of the specificity pocket. In contrast to other kallikreins, HPK shows a structurally distinct specificity pocket. Its entrance is blocked by the kallikrein loop, suggesting a possible protective or substrate-selective role for this loop. The HPK structure seems to be in an inactivated state and further processing might be required to allow the binding of substrate molecules. Crystal soaking experiments revealed a binding site for Zn(2+) and Hg(2+), two known PSA inhibitors. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Barettino, D.]] | [[Category: Barettino, D.]] | ||
| - | [[Category: Calvete, J | + | [[Category: Calvete, J J.]] |
| - | [[Category: Carvalho, A | + | [[Category: Carvalho, A L.]] |
| - | [[Category: Romao, M | + | [[Category: Romao, M J.]] |
[[Category: Romero, A.]] | [[Category: Romero, A.]] | ||
[[Category: Sanz, L.]] | [[Category: Sanz, L.]] | ||
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[[Category: prostate specific antigen]] | [[Category: prostate specific antigen]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:54:35 2008'' |
Revision as of 10:54, 21 February 2008
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PROSTATE SPECIFIC ANTIGEN (PSA) FROM STALLION SEMINAL PLASMA
Overview
Prostate-specific kallikrein, a member of the gene family of serine proteases, was initially discovered in semen and is the most useful serum marker for prostate cancer diagnosis and prognosis. We report the crystal structure at 1.42A resolution of horse prostate kallikrein (HPK). This is the first structure of a serine protease purified from seminal plasma. HPK shares extensive sequence homology with human prostate-specific antigen (PSA), including a predicted chymotrypsin-like specificity, as suggested by the presence of a serine residue at position S1 of the specificity pocket. In contrast to other kallikreins, HPK shows a structurally distinct specificity pocket. Its entrance is blocked by the kallikrein loop, suggesting a possible protective or substrate-selective role for this loop. The HPK structure seems to be in an inactivated state and further processing might be required to allow the binding of substrate molecules. Crystal soaking experiments revealed a binding site for Zn(2+) and Hg(2+), two known PSA inhibitors.
About this Structure
1GVZ is a Single protein structure of sequence from Equus caballus with and as ligands. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Crystal structure of a prostate kallikrein isolated from stallion seminal plasma: a homologue of human PSA., Carvalho AL, Sanz L, Barettino D, Romero A, Calvete JJ, Romao MJ, J Mol Biol. 2002 Sep 13;322(2):325-37. PMID:12217694
Page seeded by OCA on Thu Feb 21 12:54:35 2008
