1gwc
From Proteopedia
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==Overview== | ==Overview== | ||
| - | Glutathione S-transferases (GSTs) from the phi (GSTF) and tau (GSTU) | + | Glutathione S-transferases (GSTs) from the phi (GSTF) and tau (GSTU) classes are unique to plants and play important roles in stress tolerance and secondary metabolism as well as catalyzing the detoxification of herbicides in crops and weeds. We have cloned and functionally characterized a group of GSTUs from wheat treated with fenchlorazole-ethyl, a herbicide safener. One of these enzymes, TaGSTU4-4, was highly active in conjugating the chemically distinct wheat herbicides fenoxaprop and dimethenamid. The structure of TaGSTU4-4 has been determined at 2.2 A resolution in complex with S-hexylglutathione. This enzyme is the first tau class GST structure to be determined and most closely resembles the omega class GSTs, but without the unique N-terminal extension or active site cysteine. The X-ray structure identifies key amino acid residues in the hydrophobic binding site and provides insights into the substrate specificity of these enzymes. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Glutathione transferase]] | [[Category: Glutathione transferase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Cole, D | + | [[Category: Cole, D J.]] |
[[Category: Cummins, I.]] | [[Category: Cummins, I.]] | ||
| - | [[Category: Dixon, D | + | [[Category: Dixon, D P.]] |
[[Category: Edwards, R.]] | [[Category: Edwards, R.]] | ||
| - | [[Category: Lapthorn, A | + | [[Category: Lapthorn, A J.]] |
[[Category: Thom, R.]] | [[Category: Thom, R.]] | ||
[[Category: GTX]] | [[Category: GTX]] | ||
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[[Category: tau class]] | [[Category: tau class]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:54:42 2008'' |
Revision as of 10:54, 21 February 2008
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THE STRUCTURE OF A TAU CLASS GLUTATHIONE S-TRANSFERASE FROM WHEAT, ACTIVE IN HERBICIDE DETOXIFICATION
Overview
Glutathione S-transferases (GSTs) from the phi (GSTF) and tau (GSTU) classes are unique to plants and play important roles in stress tolerance and secondary metabolism as well as catalyzing the detoxification of herbicides in crops and weeds. We have cloned and functionally characterized a group of GSTUs from wheat treated with fenchlorazole-ethyl, a herbicide safener. One of these enzymes, TaGSTU4-4, was highly active in conjugating the chemically distinct wheat herbicides fenoxaprop and dimethenamid. The structure of TaGSTU4-4 has been determined at 2.2 A resolution in complex with S-hexylglutathione. This enzyme is the first tau class GST structure to be determined and most closely resembles the omega class GSTs, but without the unique N-terminal extension or active site cysteine. The X-ray structure identifies key amino acid residues in the hydrophobic binding site and provides insights into the substrate specificity of these enzymes.
About this Structure
1GWC is a Single protein structure of sequence from Aegilops tauschii with and as ligands. Active as Glutathione transferase, with EC number 2.5.1.18 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Structure of a tau class glutathione S-transferase from wheat active in herbicide detoxification., Thom R, Cummins I, Dixon DP, Edwards R, Cole DJ, Lapthorn AJ, Biochemistry. 2002 Jun 4;41(22):7008-20. PMID:12033934
Page seeded by OCA on Thu Feb 21 12:54:42 2008
