1h6h
From Proteopedia
| Line 4: | Line 4: | ||
==Overview== | ==Overview== | ||
| - | More than 50 human proteins with a wide range of functions have a 120 | + | More than 50 human proteins with a wide range of functions have a 120 residue phosphoinositide binding module known as the PX domain. The 1.7 A X-ray crystal structure of the PX domain from the p40(phox) subunit of NADPH oxidase bound to PtdIns(3)P shows that the PX domain embraces the 3-phosphate on one side of a water-filled, positively charged pocket and reveals how 3-phosphoinositide specificity is achieved. A chronic granulomatous disease (CGD)-associated mutation in the p47(phox) PX domain that abrogates PtdIns(3)P binding maps to a conserved Arg that does not directly interact with the phosphoinositide but instead appears to stabilize a critical lipid binding loop. The SH3 domain present in the full-length protein does not affect soluble PtdIns(3)P binding to the p40(phox) PX domain. |
==About this Structure== | ==About this Structure== | ||
| Line 17: | Line 17: | ||
[[Category: Pacold, M.]] | [[Category: Pacold, M.]] | ||
[[Category: Perisic, O.]] | [[Category: Perisic, O.]] | ||
| - | [[Category: Williams, R | + | [[Category: Williams, R L.]] |
[[Category: GOL]] | [[Category: GOL]] | ||
[[Category: PIB]] | [[Category: PIB]] | ||
[[Category: px domain]] | [[Category: px domain]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:57:56 2008'' |
Revision as of 10:57, 21 February 2008
|
STRUCTURE OF THE PX DOMAIN FROM P40PHOX BOUND TO PHOSPHATIDYLINOSITOL 3-PHOSPHATE
Overview
More than 50 human proteins with a wide range of functions have a 120 residue phosphoinositide binding module known as the PX domain. The 1.7 A X-ray crystal structure of the PX domain from the p40(phox) subunit of NADPH oxidase bound to PtdIns(3)P shows that the PX domain embraces the 3-phosphate on one side of a water-filled, positively charged pocket and reveals how 3-phosphoinositide specificity is achieved. A chronic granulomatous disease (CGD)-associated mutation in the p47(phox) PX domain that abrogates PtdIns(3)P binding maps to a conserved Arg that does not directly interact with the phosphoinositide but instead appears to stabilize a critical lipid binding loop. The SH3 domain present in the full-length protein does not affect soluble PtdIns(3)P binding to the p40(phox) PX domain.
About this Structure
1H6H is a Single protein structure of sequence from Homo sapiens with and as ligands. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
The crystal structure of the PX domain from p40(phox) bound to phosphatidylinositol 3-phosphate., Bravo J, Karathanassis D, Pacold CM, Pacold ME, Ellson CD, Anderson KE, Butler PJ, Lavenir I, Perisic O, Hawkins PT, Stephens L, Williams RL, Mol Cell. 2001 Oct;8(4):829-39. PMID:11684018
Page seeded by OCA on Thu Feb 21 12:57:56 2008
Categories: Homo sapiens | Single protein | Bravo, J. | Karathanassis, D. | Pacold, M. | Perisic, O. | Williams, R L. | GOL | PIB | Px domain
