3tfz

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[[Image:3tfz.png|left|200px]]
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==Crystal structure of Zhui aromatase/cyclase from Streptomcyes sp. R1128==
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<StructureSection load='3tfz' size='340' side='right' caption='[[3tfz]], [[Resolution|resolution]] 2.39&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3tfz]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_sp._r1128 Streptomyces sp. r1128]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TFZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TFZ FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CXS:3-CYCLOHEXYL-1-PROPYLSULFONIC+ACID'>CXS</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene><br>
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<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">zhuI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=140437 Streptomyces sp. R1128])</td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tfz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tfz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tfz RCSB], [http://www.ebi.ac.uk/pdbsum/3tfz PDBsum]</span></td></tr>
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<table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Aromatic polyketides comprise an important class of natural products that possess a wide range of biological activities. The cyclization of the polyketide chain is a critical control point in the biosynthesis of aromatic polyketides. The aromatase/cyclases (ARO/CYCs) are an important component of the type II polyketide synthase (PKS) and help fold the polyketide for regiospecific cyclizations of the first ring and/or aromatization, promoting two commonly observed first-ring cyclization patterns for the bacterial type II PKSs: C7-C12 and C9-C14. We had previously reported the crystal structure and enzymological analyses of the TcmN ARO/CYC, which promotes C9-C14 first-ring cyclization. However, how C7-C12 first-ring cyclization is controlled remains unresolved. In this work, we present the 2.4 A crystal structure of ZhuI, a C7-C12-specific first-ring ARO/CYC from the type II PKS pathway responsible for the production of the R1128 polyketides. Though ZhuI possesses a helix-grip fold shared by TcmN ARO/CYC, there are substantial differences in overall structure and pocket residue composition that may be important for directing C7-C12 (rather than C9-C14) cyclization. Docking studies and site-directed mutagenesis coupled to an in vitro activity assay demonstrate that ZhuI pocket residues R66, H109, and D146 are important for enzyme function. The ZhuI crystal structure helps visualize the structure and putative dehydratase function of the didomain ARO/CYCs from KR-containing type II PKSs. The sequence-structure-function analysis described for ZhuI elucidates the molecular mechanisms that control C7-C12 first-ring polyketide cyclization and builds a foundation for future endeavors into directing cyclization patterns for engineered biosynthesis of aromatic polyketides.
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Structural and Biochemical Characterization of ZhuI Aromatase/Cyclase from the R1128 Polyketide Pathway.,Ames BD, Lee MY, Moody C, Zhang W, Tang Y, Tsai SC Biochemistry. 2011 Sep 8. PMID:21870821<ref>PMID:21870821</ref>
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The line below this paragraph, containing "STRUCTURE_3tfz", creates the "Structure Box" on the page.
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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or leave the SCENE parameter empty for the default display.
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{{STRUCTURE_3tfz| PDB=3tfz | SCENE= }}
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===Crystal structure of Zhui aromatase/cyclase from Streptomcyes sp. R1128===
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
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<references/>
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The line below this paragraph, {{ABSTRACT_PUBMED_21870821}}, adds the Publication Abstract to the page
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__TOC__
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(as it appears on PubMed at http://www.pubmed.gov), where 21870821 is the PubMed ID number.
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</StructureSection>
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{{ABSTRACT_PUBMED_21870821}}
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==About this Structure==
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[[3tfz]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_sp._r1128 Streptomyces sp. r1128]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TFZ OCA].
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==Reference==
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<ref group="xtra">PMID:021870821</ref><references group="xtra"/>
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[[Category: Streptomyces sp. r1128]]
[[Category: Streptomyces sp. r1128]]
[[Category: Ames, B D.]]
[[Category: Ames, B D.]]

Revision as of 05:40, 5 June 2014

Crystal structure of Zhui aromatase/cyclase from Streptomcyes sp. R1128

3tfz, resolution 2.39Å

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