1h8e
From Proteopedia
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==Overview== | ==Overview== | ||
| - | The crystal structure of a novel aluminium fluoride inhibited form of | + | The crystal structure of a novel aluminium fluoride inhibited form of bovine mitochondrial F(1)-ATPase has been determined at 2 A resolution. In contrast to all previously determined structures of the bovine enzyme, all three catalytic sites are occupied by nucleotide. The subunit that did not bind nucleotide in previous structures binds ADP and sulfate (mimicking phosphate), and adopts a "half-closed" conformation. This structure probably represents the posthydrolysis, pre-product release step on the catalytic pathway. A catalytic scheme for hydrolysis (and synthesis) at physiological rates and a mechanism for the ATP-driven rotation of the gamma subunit are proposed based on the crystal structures of the bovine enzyme. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Bos taurus]] | [[Category: Bos taurus]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Transferred entry: 3 | + | [[Category: Transferred entry: 3 6.3 14]] |
| - | [[Category: Leslie, A | + | [[Category: Leslie, A G.W.]] |
| - | [[Category: Menz, R | + | [[Category: Menz, R I.]] |
| - | [[Category: Walker, J | + | [[Category: Walker, J E.]] |
[[Category: ADP]] | [[Category: ADP]] | ||
[[Category: ALF]] | [[Category: ALF]] | ||
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[[Category: f1fo atp synthase]] | [[Category: f1fo atp synthase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:58:33 2008'' |
Revision as of 10:58, 21 February 2008
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(ADP.ALF4)2(ADP.SO4) BOVINE F1-ATPASE (ALL THREE CATALYTIC SITES OCCUPIED)
Overview
The crystal structure of a novel aluminium fluoride inhibited form of bovine mitochondrial F(1)-ATPase has been determined at 2 A resolution. In contrast to all previously determined structures of the bovine enzyme, all three catalytic sites are occupied by nucleotide. The subunit that did not bind nucleotide in previous structures binds ADP and sulfate (mimicking phosphate), and adopts a "half-closed" conformation. This structure probably represents the posthydrolysis, pre-product release step on the catalytic pathway. A catalytic scheme for hydrolysis (and synthesis) at physiological rates and a mechanism for the ATP-driven rotation of the gamma subunit are proposed based on the crystal structures of the bovine enzyme.
About this Structure
1H8E is a Protein complex structure of sequences from Bos taurus with , , , and as ligands. Active as Transferred entry: 3.6.3.14, with EC number 3.6.1.34 Known structural/functional Sites: , , , , , , , , , , , , , , , and . Full crystallographic information is available from OCA.
Reference
Structure of bovine mitochondrial F(1)-ATPase with nucleotide bound to all three catalytic sites: implications for the mechanism of rotary catalysis., Menz RI, Walker JE, Leslie AG, Cell. 2001 Aug 10;106(3):331-41. PMID:11509182
Page seeded by OCA on Thu Feb 21 12:58:33 2008
