1ldz
From Proteopedia
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==Overview== | ==Overview== | ||
| - | The NMR solution structure of a lead-dependent ribozyme, known as the | + | The NMR solution structure of a lead-dependent ribozyme, known as the leadzyme, is presented. This ribozyme is among the smallest of the known catalytic RNAs, with an active site consisting of a six-nucleotide asymmetric internal loop. This loop has a roughly double-helical structure, including a protonated adenine-cytosine wobble base-pair, that positions the cytosine base 5' to the cleavage site in a double-helical conformation. The deviations from helical structure consist of two bulged guanosine residues, G7 and G9, where G7 is the residue 3' to the cleavage site. The scissile phosphate group of the leadzyme is not positioned for in-line nucleophilic attack. Therefore, a conformational rearrangement in the active site is required to reach the proposed transition state for this ribozyme. This is similar to previous observations in X-ray studies of the hammerhead ribozyme, and emphasizes the necessity for dynamic structural fluctuations in the catalytic mechanism of small ribozymes. A model for metal-binding in the leadzyme is proposed in which a lead ion binds to a bulged guanine base that is critical for leadzyme function. |
==About this Structure== | ==About this Structure== | ||
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NMR solution structure of the lead-dependent ribozyme: evidence for dynamics in RNA catalysis., Hoogstraten CG, Legault P, Pardi A, J Mol Biol. 1998 Nov 27;284(2):337-50. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9813122 9813122] | NMR solution structure of the lead-dependent ribozyme: evidence for dynamics in RNA catalysis., Hoogstraten CG, Legault P, Pardi A, J Mol Biol. 1998 Nov 27;284(2):337-50. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9813122 9813122] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Hoogstraten, C | + | [[Category: Hoogstraten, C G.]] |
[[Category: Legault, P.]] | [[Category: Legault, P.]] | ||
[[Category: Pardi, A.]] | [[Category: Pardi, A.]] | ||
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[[Category: rna structure]] | [[Category: rna structure]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:44:04 2008'' |
Revision as of 11:44, 21 February 2008
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SOLUTION STRUCTURE OF THE LEAD-DEPENDENT RIBOZYME, NMR, 25 STRUCTURES
Overview
The NMR solution structure of a lead-dependent ribozyme, known as the leadzyme, is presented. This ribozyme is among the smallest of the known catalytic RNAs, with an active site consisting of a six-nucleotide asymmetric internal loop. This loop has a roughly double-helical structure, including a protonated adenine-cytosine wobble base-pair, that positions the cytosine base 5' to the cleavage site in a double-helical conformation. The deviations from helical structure consist of two bulged guanosine residues, G7 and G9, where G7 is the residue 3' to the cleavage site. The scissile phosphate group of the leadzyme is not positioned for in-line nucleophilic attack. Therefore, a conformational rearrangement in the active site is required to reach the proposed transition state for this ribozyme. This is similar to previous observations in X-ray studies of the hammerhead ribozyme, and emphasizes the necessity for dynamic structural fluctuations in the catalytic mechanism of small ribozymes. A model for metal-binding in the leadzyme is proposed in which a lead ion binds to a bulged guanine base that is critical for leadzyme function.
About this Structure
1LDZ is a Protein complex structure of sequences from [1]. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
NMR solution structure of the lead-dependent ribozyme: evidence for dynamics in RNA catalysis., Hoogstraten CG, Legault P, Pardi A, J Mol Biol. 1998 Nov 27;284(2):337-50. PMID:9813122
Page seeded by OCA on Thu Feb 21 13:44:04 2008
