1oek
From Proteopedia
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==Overview== | ==Overview== | ||
| - | We have determined the crystal structure of YodA, an Escherichia coli | + | We have determined the crystal structure of YodA, an Escherichia coli protein of unknown function. YodA had been identified under conditions of cadmium stress, and we confirm that it binds metals such as cadmium and zinc. We have also found nickel bound in one of the crystal forms. YodA is composed of two domains: a main lipocalin/calycin-like domain and a helical domain. The principal metal-binding site lies on one side of the calycin domain, thus making YodA the first metal-binding lipocalin known. Our experiments suggest that YodA expression may be part of a more general stress response. From sequence analogy with the C-terminal domain of a metal-binding receptor of a member of bacterial ATP-binding cassette transporters, we propose a three-dimensional model for this receptor and suggest that YodA may have a receptor-type partner in E. coli. |
==About this Structure== | ==About this Structure== | ||
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[[Category: yoda]] | [[Category: yoda]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:16:47 2008'' |
Revision as of 12:16, 21 February 2008
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YODA FROM ESCHERICHIA COLI CRYSTALLISED WITH ZINC IONS
Overview
We have determined the crystal structure of YodA, an Escherichia coli protein of unknown function. YodA had been identified under conditions of cadmium stress, and we confirm that it binds metals such as cadmium and zinc. We have also found nickel bound in one of the crystal forms. YodA is composed of two domains: a main lipocalin/calycin-like domain and a helical domain. The principal metal-binding site lies on one side of the calycin domain, thus making YodA the first metal-binding lipocalin known. Our experiments suggest that YodA expression may be part of a more general stress response. From sequence analogy with the C-terminal domain of a metal-binding receptor of a member of bacterial ATP-binding cassette transporters, we propose a three-dimensional model for this receptor and suggest that YodA may have a receptor-type partner in E. coli.
About this Structure
1OEK is a Single protein structure of sequence from Escherichia coli with as ligand. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
YodA from Escherichia coli is a metal-binding, lipocalin-like protein., David G, Blondeau K, Schiltz M, Penel S, Lewit-Bentley A, J Biol Chem. 2003 Oct 31;278(44):43728-35. Epub 2003 Aug 8. PMID:12909634
Page seeded by OCA on Thu Feb 21 14:16:47 2008
Categories: Escherichia coli | Single protein | Blondeau, K. | David, G. | Lewit-Bentley, A. | Penel, S. | Renouard, M. | ZN | Lipocalin | Stress protein | Yoda
