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1qny

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==Overview==
==Overview==
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The correct positions of the deuterium (D) atoms of many of the bound, waters in the protein concanavalin A are revealed by neutron Laue, diffraction. The approach includes cases where these water D atoms show, enough mobility to render them invisible even to ultra-high resolution, synchrotron-radiation X-ray crystallography. The positions of the bound, water H atoms calculated on the basis of chemical and energetic, considerations are often incorrect. The D-atom positions for the water, molecules in the Mn-, Ca- and sugar-binding sites of concanavalin A are, described in detail.
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The correct positions of the deuterium (D) atoms of many of the bound waters in the protein concanavalin A are revealed by neutron Laue diffraction. The approach includes cases where these water D atoms show enough mobility to render them invisible even to ultra-high resolution synchrotron-radiation X-ray crystallography. The positions of the bound water H atoms calculated on the basis of chemical and energetic considerations are often incorrect. The D-atom positions for the water molecules in the Mn-, Ca- and sugar-binding sites of concanavalin A are described in detail.
==About this Structure==
==About this Structure==
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[[Category: Canavalia ensiformis]]
[[Category: Canavalia ensiformis]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Gilboa, A.J.Kalb.]]
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[[Category: Gilboa, A J.Kalb.]]
[[Category: Habash, J.]]
[[Category: Habash, J.]]
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[[Category: Helliwell, J.R.]]
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[[Category: Helliwell, J R.]]
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[[Category: Lehmann, M.S.]]
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[[Category: Lehmann, M S.]]
[[Category: Nuttall, R.]]
[[Category: Nuttall, R.]]
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[[Category: Price, H.J.]]
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[[Category: Price, H J.]]
[[Category: Raftery, J.]]
[[Category: Raftery, J.]]
[[Category: Wilkinson, C.]]
[[Category: Wilkinson, C.]]
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[[Category: sacchsaride free]]
[[Category: sacchsaride free]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:01:24 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:41:46 2008''

Revision as of 12:41, 21 February 2008


1qny, resolution 1.8Å

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X-RAY REFINEMENT OF D2O SOAKED CRYSTAL OF CONCANAVALIN A

Overview

The correct positions of the deuterium (D) atoms of many of the bound waters in the protein concanavalin A are revealed by neutron Laue diffraction. The approach includes cases where these water D atoms show enough mobility to render them invisible even to ultra-high resolution synchrotron-radiation X-ray crystallography. The positions of the bound water H atoms calculated on the basis of chemical and energetic considerations are often incorrect. The D-atom positions for the water molecules in the Mn-, Ca- and sugar-binding sites of concanavalin A are described in detail.

About this Structure

1QNY is a Single protein structure of sequence from Canavalia ensiformis with and as ligands. Known structural/functional Sites: and . Full crystallographic information is available from OCA.

Reference

Direct determination of the positions of the deuterium atoms of the bound water in -concanavalin A by neutron Laue crystallography., Habash J, Raftery J, Nuttall R, Price HJ, Wilkinson C, Kalb AJ, Helliwell JR, Acta Crystallogr D Biol Crystallogr. 2000 May;56(Pt 5):541-50. PMID:10771422

Page seeded by OCA on Thu Feb 21 14:41:46 2008

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