Sandbox 40

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<scene name='Sandbox_40/Lipase_qm/2'>polar and nonpolar residues</scene>. The polar residues in this scene are shown in a light blue shade, and the nonpolar are in a dark red. From this representation, it can be assumed that there is a similar quantity of polar residues as there are nonopolar. The tertiary stucture of the molecule is stabilized by 6 <scene name='Sandbox_40/Disulfide_bonds/1'>disulfide bonds</scene> and ionic interactions with a calcium ligand. Finally, the quaternary structure is completed by the adjoining of the two identical subunits.
<scene name='Sandbox_40/Lipase_qm/2'>polar and nonpolar residues</scene>. The polar residues in this scene are shown in a light blue shade, and the nonpolar are in a dark red. From this representation, it can be assumed that there is a similar quantity of polar residues as there are nonopolar. The tertiary stucture of the molecule is stabilized by 6 <scene name='Sandbox_40/Disulfide_bonds/1'>disulfide bonds</scene> and ionic interactions with a calcium ligand. Finally, the quaternary structure is completed by the adjoining of the two identical subunits.
=The Calcium Ligand=
=The Calcium Ligand=
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The <scene name='Sandbox_40/Lipase_ligand/1'>ligands of lipase</scene> are two calcium ions, one buried within each subunit.
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The <scene name='Sandbox_40/Lipase_ligand/1'>ligands of lipase</scene> are two calcium ions, one buried within each subunit. This scene shows the interactions between the calcium ion and the following residues: GLU187, ARG190, ASP192, and ASP195. In addition to interactions with these molecules, the calcium ion is also stabilized by the oxygens from two water molecules shown in pink.
=The Mechanism=
=The Mechanism=
[[Image:Hydrolysis of trigly.png|200px|left|thumb| This is the reaction catalyzed by most lipases. Shown is a triglyceride being hydrolyzed by water, resulting in triglycerol and three fatty acids.]]
[[Image:Hydrolysis of trigly.png|200px|left|thumb| This is the reaction catalyzed by most lipases. Shown is a triglyceride being hydrolyzed by water, resulting in triglycerol and three fatty acids.]]
<scene name='Sandbox_40/Active_site/1'>Active Site</scene>
<scene name='Sandbox_40/Active_site/1'>Active Site</scene>

Revision as of 02:09, 14 November 2011

Please do NOT make changes to this Sandbox. Sandboxes 30-60 are reserved for use by Biochemistry 410 & 412 at Messiah College taught by Dr. Hannah Tims during Fall 2012 and Spring 2013.

Horse Pancreatic Lipase

Structure of Horse Pancreatic Lipase (PDB entry 1hpl)

Drag the structure with the mouse to rotate
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