User:Mitchell Long/Sandbox 1

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Revision as of 03:27, 17 November 2011

spinqualitylabelsall models PDB ID 3fgc Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image Introduction Luciferases are a class of enzymes that catalyze the oxidation of a long chain aliphatic aldehydes. This reaction results in the formation of a carboxylic acid and the emission of photons in the form of blue-green light.

spinqualitylabelsall models PDB ID 3fgc Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image
3fgc, resolution 2.30Å ()
Ligands:	, ,
Gene:	luxA (Vibrio harveyi), luxB (Vibrio harveyi)
Activity:	Alkanal monooxygenase (FMN-linked), with EC number 1.14.14.3
Related:	1luc, 1brl
Structural annotation: Resources: InterPro : Ipr011251, Ipr016048, Ipr002103, Ipr018235 Pfam : PF00296 UniProt : P07740, P07739
Evolutionary conservation: Toggle Conservation Colors: Rows = identical sequences: Further Information: Caveats, Explanation, Complete Results at ConSurfDB
Resources:	FirstGlance, OCA, RCSB, PDBsum
Coordinates:	save as pdb, mmCIF, xml

Contents 1 Mechanism of Bio luminescence 2 Structural Motifs 3 Applications In Biotechnology 4 Quorum Sensing 5 References

Mechanism of Bio luminescence

Luciferase found in 'Vibrio harveyi' is a heterodimer that is composed of a catalytic α subunit and a homologous but noncatalytic beta subunit. The catalytic

Structural Motifs

Active Site

Mobile Loop (β/α)₈ Barrel Interface between Alpha and Beta Subunits

Applications In Biotechnology

The luciferase assay

Quorum Sensing

Quorum sensing is a population density dependent form gene expression found in many bacteria.

References