2bf0
From Proteopedia
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==Overview== | ==Overview== | ||
| - | The C-terminal domain (CTD) of the large subunit of RNA polymerase II is a | + | The C-terminal domain (CTD) of the large subunit of RNA polymerase II is a platform for mRNA processing factors and links gene transcription to mRNA capping, splicing and polyadenylation. Pcf11, an essential component of the mRNA cleavage factor IA, contains a CTD-interaction domain that binds in a phospho-dependent manner to the heptad repeats within the RNA polymerase II CTD. We show here that the phosphorylated CTD exists as a dynamic disordered ensemble in solution and, by induced fit, it assumes a structured conformation when bound to Pcf11. In addition, we detected cis-trans populations for the CTD prolines, and found that only the all-trans form is selected for binding. These data suggest that the recognition of the CTD is regulated by independent site-specific modifications (phosphorylation and proline cis-trans isomerization) and, probably, by the local concentration of suitable binding sites. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Adeniran, V | + | [[Category: Adeniran, V E.]] |
[[Category: Hollingworth, D.]] | [[Category: Hollingworth, D.]] | ||
[[Category: Kelly, G.]] | [[Category: Kelly, G.]] | ||
| - | [[Category: Martin, S | + | [[Category: Martin, S R.]] |
| - | [[Category: Noble, C | + | [[Category: Noble, C G.]] |
[[Category: Ramos, A.]] | [[Category: Ramos, A.]] | ||
| - | [[Category: Smerdon, S | + | [[Category: Smerdon, S J.]] |
| - | [[Category: Taylor, I | + | [[Category: Taylor, I A.]] |
[[Category: CA]] | [[Category: CA]] | ||
[[Category: phosphorylation]] | [[Category: phosphorylation]] | ||
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[[Category: transcription]] | [[Category: transcription]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:37:09 2008'' |
Revision as of 14:37, 21 February 2008
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CRYSTAL STRUCTURE OF THE RPR OF PCF11
Overview
The C-terminal domain (CTD) of the large subunit of RNA polymerase II is a platform for mRNA processing factors and links gene transcription to mRNA capping, splicing and polyadenylation. Pcf11, an essential component of the mRNA cleavage factor IA, contains a CTD-interaction domain that binds in a phospho-dependent manner to the heptad repeats within the RNA polymerase II CTD. We show here that the phosphorylated CTD exists as a dynamic disordered ensemble in solution and, by induced fit, it assumes a structured conformation when bound to Pcf11. In addition, we detected cis-trans populations for the CTD prolines, and found that only the all-trans form is selected for binding. These data suggest that the recognition of the CTD is regulated by independent site-specific modifications (phosphorylation and proline cis-trans isomerization) and, probably, by the local concentration of suitable binding sites.
About this Structure
2BF0 is a Single protein structure of sequence from Saccharomyces cerevisiae with as ligand. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Key features of the interaction between Pcf11 CID and RNA polymerase II CTD., Noble CG, Hollingworth D, Martin SR, Ennis-Adeniran V, Smerdon SJ, Kelly G, Taylor IA, Ramos A, Nat Struct Mol Biol. 2005 Feb;12(2):144-51. Epub 2005 Jan 16. PMID:15665873
Page seeded by OCA on Thu Feb 21 16:37:09 2008
