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2bfq

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==Overview==
==Overview==
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The ADP-ribosylation of proteins is an important post-translational, modification that occurs in a variety of biological processes, including, DNA repair, transcription, chromatin biology and long-term memory, formation. Yet no protein modules are known that specifically recognize, the ADP-ribose nucleotide. We provide biochemical and structural evidence, that macro domains are high-affinity ADP-ribose binding modules. Our, structural analysis reveals a conserved ligand binding pocket among the, macro domain fold. Consistently, distinct human macro domains retain their, ability to bind ADP-ribose. In addition, some macro domain proteins also, recognize poly-ADP-ribose as a ligand. Our data suggest an important role, for proteins containing macro domains in the biology of ADP-ribose.
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The ADP-ribosylation of proteins is an important post-translational modification that occurs in a variety of biological processes, including DNA repair, transcription, chromatin biology and long-term memory formation. Yet no protein modules are known that specifically recognize the ADP-ribose nucleotide. We provide biochemical and structural evidence that macro domains are high-affinity ADP-ribose binding modules. Our structural analysis reveals a conserved ligand binding pocket among the macro domain fold. Consistently, distinct human macro domains retain their ability to bind ADP-ribose. In addition, some macro domain proteins also recognize poly-ADP-ribose as a ligand. Our data suggest an important role for proteins containing macro domains in the biology of ADP-ribose.
==About this Structure==
==About this Structure==
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[[Category: Archaeoglobus fulgidus]]
[[Category: Archaeoglobus fulgidus]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Allen, M.D.]]
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[[Category: Allen, M D.]]
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[[Category: Buhecha, H.R.]]
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[[Category: Buhecha, H R.]]
[[Category: Bycroft, M.]]
[[Category: Bycroft, M.]]
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[[Category: Karras, G.I.]]
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[[Category: Karras, G I.]]
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[[Category: Ladurner, A.G.]]
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[[Category: Ladurner, A G.]]
[[Category: Pugieux, C.]]
[[Category: Pugieux, C.]]
[[Category: Sait, F.]]
[[Category: Sait, F.]]
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[[Category: nucleotide]]
[[Category: nucleotide]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:23:34 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:37:20 2008''

Revision as of 14:37, 21 February 2008


2bfq, resolution 1.5Å

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MACRO DOMAINS ARE ADP-RIBOSE BINDING MOLECULES

Overview

The ADP-ribosylation of proteins is an important post-translational modification that occurs in a variety of biological processes, including DNA repair, transcription, chromatin biology and long-term memory formation. Yet no protein modules are known that specifically recognize the ADP-ribose nucleotide. We provide biochemical and structural evidence that macro domains are high-affinity ADP-ribose binding modules. Our structural analysis reveals a conserved ligand binding pocket among the macro domain fold. Consistently, distinct human macro domains retain their ability to bind ADP-ribose. In addition, some macro domain proteins also recognize poly-ADP-ribose as a ligand. Our data suggest an important role for proteins containing macro domains in the biology of ADP-ribose.

About this Structure

2BFQ is a Single protein structure of sequence from Archaeoglobus fulgidus with as ligand. Known structural/functional Site: . Full crystallographic information is available from OCA.

Reference

The macro domain is an ADP-ribose binding module., Karras GI, Kustatscher G, Buhecha HR, Allen MD, Pugieux C, Sait F, Bycroft M, Ladurner AG, EMBO J. 2005 Jun 1;24(11):1911-20. Epub 2005 May 19. PMID:15902274

Page seeded by OCA on Thu Feb 21 16:37:20 2008

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