DNA methyltransferase
From Proteopedia
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{{STRUCTURE_1qnt| PDB=1qnt | SIZE=400| SCENE= |right|CAPTION=Human O6-methylguanine DNA methyltransferase, [[1qnt]] }} | {{STRUCTURE_1qnt| PDB=1qnt | SIZE=400| SCENE= |right|CAPTION=Human O6-methylguanine DNA methyltransferase, [[1qnt]] }} | ||
| - | '''DNA methyltransferase''' (DNMT) catalyzes the transfer of methyl group to DNA. DNMT uses S-adenosyl methionine (SAM) as the methyl donor. | + | '''DNA methyltransferase''' (DNMT) catalyzes the transfer of methyl group to DNA. DNMT uses S-adenosyl methionine (SAM) as the methyl donor. The SAM analog S-adenosyl homocysteine (SAH) is a powerful inhibitor of DNMT. |
{{TOC limit|limit=2}} | {{TOC limit|limit=2}} | ||
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== 3D Structures of DNA methyltransferase == | == 3D Structures of DNA methyltransferase == | ||
| - | ''Updated | + | ''Updated June 2012'' |
===Methylguanine DNMT=== | ===Methylguanine DNMT=== | ||
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[[3a1a]] - h-cytosine DNMT PWWP domain | [[3a1a]] - h-cytosine DNMT PWWP domain | ||
| - | [[1g55]] - h-cytosine DNMT2 (mutant) | + | [[1g55]] - h-cytosine DNMT2 (mutant)<br /> |
| - | + | [[3qkj[[ - h-cytosine DNMT 3β<br /> | |
[[3av4]], [[1khc]] - m-cytosine DNMT – mouse | [[3av4]], [[1khc]] - m-cytosine DNMT – mouse | ||
| - | + | [[2uyc]], [[2uyh]], [[2uz4]] - Hh-cytosine DNMT (mutant) + DNA + SAH | |
| - | + | ||
| - | [[2uyc]], [[2uyh]], [[2uz4]] - Hh-cytosine DNMT (mutant) + DNA + | + | |
[[3a1b]] - h-cytosine DNMT ADD domain + histone H3 | [[3a1b]] - h-cytosine DNMT ADD domain + histone H3 | ||
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[[3pt6]], [[3pt9]] – m-cytosine DNMT + DNA | [[3pt6]], [[3pt9]] – m-cytosine DNMT + DNA | ||
| - | [[3av5]] - m-cytosine DNMT + | + | [[3av5]] - m-cytosine DNMT + SAH |
[[3av6]] - m-cytosine DNMT + SAM | [[3av6]] - m-cytosine DNMT + SAM | ||
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[[3swr]] - h-cytosine DNMT + sinefungin | [[3swr]] - h-cytosine DNMT + sinefungin | ||
| - | [[1boo]] - cytosine DNMT (mutant) – ''Proteus vulgaris'' | + | [[1boo]] - cytosine DNMT (mutant) – ''Proteus vulgaris''<br /> |
| + | [[3qv2]] - cytosine DNMT + SAH – ''Entamoeba histolytica''<br /> | ||
| + | [[2uyc]], [[2uyh]], [[2uz4]], [[1fjx]] – Hh-cytosine DNMT (mutant) + DNA + SAH - ''Haemophilus haemolyticus''<br /> | ||
| + | [[2c7o]], [[2c7p]], [[2c7q]], [[2c7r]], [[3pta]] – Hh-cytosine DNMT + DNA + S-adenonosyl cysteine<br /> | ||
| + | [[1hmy]] – Hh-cytosine Haeiii DNMT + SAM | ||
| - | [[1fjx]] – HhDNMT (mutant) + DNA + S-adenonosyl cysteine - ''Haemophilus haemolyticus'' | ||
| - | [[ | + | [[1m0e]] - Hh-cytosine DNMT (mutant) + DNA + inhibitor |
| + | [[2i9k]] - Hh-cytosine DNMT (mutant) + DNA | ||
| - | [[ | + | [[2hr1]] - Hh-cytosine DNMT + DNA + ADOHCY |
| - | [[ | + | [[2z6q]], [[2z6u]], [[2zcj]] - Hh-cytosine DNMT (mutant) + DNA + ADOHCY |
| - | [[ | + | [[1svu]] - Hh-cytosine DNMT (mutant) |
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
===Methyladenine DNMT=== | ===Methyladenine DNMT=== | ||
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[[1nw5]] – RsN6-adenine DNMT + SAM – ''Rhodobacter sphaeroides'' | [[1nw5]] – RsN6-adenine DNMT + SAM – ''Rhodobacter sphaeroides'' | ||
| - | [[1nw6]] - RsN6-adenine DNMT + sinefungin | + | [[1nw6]] - RsN6-adenine DNMT + sinefungin<br /> |
| - | + | [[1nw7]] - RsN6-adenine DNMT + SAH<br /> | |
[[1nw8]] - RsN6-adenine DNMT (mutant) | [[1nw8]] - RsN6-adenine DNMT (mutant) | ||
| - | [[2ibs]], [[2ibt]], [[2ih2]], [[2ih4]], [[2ih5]], [[2np6]], [[2np7]] - adenine DNMT Taqi + DNA + cofactor analog – ''Thermus aquaticus'' | + | [[2ibs]], [[2ibt]], [[2ih2]], [[2ih4]], [[2ih5]], [[2np6]], [[2np7]], [[1g38]] - Ta-adenine DNMT Taqi + DNA + cofactor analog – ''Thermus aquaticus''<br /> |
| + | [[1aqi]] – Ta-adenine DNMT Taqi + SAH<br /> | ||
| + | [[1aqj]] – Ta-adenine DNMT Taqi + sinefugin<br /> | ||
| + | [[2adm]] - Ta-adenine DNMT Taqi + SAM<br /> | ||
| + | [[1g60]] - adenine DNMT + SAM – ''Moraxella bovis'' | ||
| + | |||
| - | [[1nw7]] - RsN6-adenine DNMT + S-adenonosyl cysteine | ||
[[Category:Topic Page]] | [[Category:Topic Page]] | ||
Revision as of 06:46, 7 June 2012
DNA methyltransferase (DNMT) catalyzes the transfer of methyl group to DNA. DNMT uses S-adenosyl methionine (SAM) as the methyl donor. The SAM analog S-adenosyl homocysteine (SAH) is a powerful inhibitor of DNMT.
Contents |
3D Structures of DNA methyltransferase
Updated June 2012
Methylguanine DNMT
1sfe – O6-methylguanine DNMT C terminal – Escherichia coli
1qnt, 1eh6, 3llr - hO6-methylguanine DNMT – human
1t38 - hO6-methylguanine DNMT (mutant) + DNA
1t39, 1yfh - hO6-methylguanine DNMT + DNA
1eh7 - hO6-methylguanine DNMT methylated
1eh8 - hO6-methylguanine DNMT benzylated
1wrj - O6-methylguanine DNMT - Sulfolobus tokodaii
2g7h - O6-methylguanine DNMT – Methanocaldococcus jannaschii
2kif, 2kim - O6-methylguanine DNMT – Vibrio parahaemolyticus - NMR
Methylcytosine DNMT
2qrv - h-cytosine DNMT
3epz - h-cytosine DNMT replecation foci-targeting sequence
3flg - h-cytosine DNMT ADD domain
3a1a - h-cytosine DNMT PWWP domain
1g55 - h-cytosine DNMT2 (mutant)
[[3qkj[[ - h-cytosine DNMT 3β
3av4, 1khc - m-cytosine DNMT – mouse
2uyc, 2uyh, 2uz4 - Hh-cytosine DNMT (mutant) + DNA + SAH
3a1b - h-cytosine DNMT ADD domain + histone H3
3pt6, 3pt9 – m-cytosine DNMT + DNA
3av5 - m-cytosine DNMT + SAH
3av6 - m-cytosine DNMT + SAM
3swr - h-cytosine DNMT + sinefungin
1boo - cytosine DNMT (mutant) – Proteus vulgaris
3qv2 - cytosine DNMT + SAH – Entamoeba histolytica
2uyc, 2uyh, 2uz4, 1fjx – Hh-cytosine DNMT (mutant) + DNA + SAH - Haemophilus haemolyticus
2c7o, 2c7p, 2c7q, 2c7r, 3pta – Hh-cytosine DNMT + DNA + S-adenonosyl cysteine
1hmy – Hh-cytosine Haeiii DNMT + SAM
1m0e - Hh-cytosine DNMT (mutant) + DNA + inhibitor
2i9k - Hh-cytosine DNMT (mutant) + DNA
2hr1 - Hh-cytosine DNMT + DNA + ADOHCY
2z6q, 2z6u, 2zcj - Hh-cytosine DNMT (mutant) + DNA + ADOHCY
1svu - Hh-cytosine DNMT (mutant)
Methyladenine DNMT
1nw5 – RsN6-adenine DNMT + SAM – Rhodobacter sphaeroides
1nw6 - RsN6-adenine DNMT + sinefungin
1nw7 - RsN6-adenine DNMT + SAH
1nw8 - RsN6-adenine DNMT (mutant)
2ibs, 2ibt, 2ih2, 2ih4, 2ih5, 2np6, 2np7, 1g38 - Ta-adenine DNMT Taqi + DNA + cofactor analog – Thermus aquaticus
1aqi – Ta-adenine DNMT Taqi + SAH
1aqj – Ta-adenine DNMT Taqi + sinefugin
2adm - Ta-adenine DNMT Taqi + SAM
1g60 - adenine DNMT + SAM – Moraxella bovis
