2bp6
From Proteopedia
| Line 4: | Line 4: | ||
==Overview== | ==Overview== | ||
| - | The lectin from Pseudomonas aeruginosa (PA-IIL) is involved in host | + | The lectin from Pseudomonas aeruginosa (PA-IIL) is involved in host recognition and biofilm formation. Lectin not only displays an unusually high affinity for fucose but also binds to L-fucose, L-galactose and D-arabinose that differ only by the group at position 5 of the sugar ring. Isothermal calorimetry experiments provided precise determination of affinity for the three methyl-glycosides and revealed a large enthalpy contribution. The crystal structures of the complexes of PA-IIL with L-galactose and Met-beta-D-arabinoside have been determined and compared with the PA-IIL/fucose complex described previously. A combination of the structures and thermodynamics provided clues for the role of the hydrophobic group in affinity. |
==About this Structure== | ==About this Structure== | ||
| Line 14: | Line 14: | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Imberty, A.]] | [[Category: Imberty, A.]] | ||
| - | [[Category: Mitchell, E | + | [[Category: Mitchell, E P.]] |
| - | [[Category: Sabin, C | + | [[Category: Sabin, C D.]] |
[[Category: Wimmerova, M.]] | [[Category: Wimmerova, M.]] | ||
[[Category: CA]] | [[Category: CA]] | ||
| Line 25: | Line 25: | ||
[[Category: pseudomonas aeruginosa]] | [[Category: pseudomonas aeruginosa]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:40:08 2008'' |
Revision as of 14:40, 21 February 2008
|
CRYSTAL STRUCTURE OF PSEUDOMONAS AERUGINOSA LECTIN (PA-IIL) COMPLEXED WITH A-L-GALACTOPYRANOSIDE
Overview
The lectin from Pseudomonas aeruginosa (PA-IIL) is involved in host recognition and biofilm formation. Lectin not only displays an unusually high affinity for fucose but also binds to L-fucose, L-galactose and D-arabinose that differ only by the group at position 5 of the sugar ring. Isothermal calorimetry experiments provided precise determination of affinity for the three methyl-glycosides and revealed a large enthalpy contribution. The crystal structures of the complexes of PA-IIL with L-galactose and Met-beta-D-arabinoside have been determined and compared with the PA-IIL/fucose complex described previously. A combination of the structures and thermodynamics provided clues for the role of the hydrophobic group in affinity.
About this Structure
2BP6 is a Single protein structure of sequence from Pseudomonas aeruginosa with , and as ligands. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Binding of different monosaccharides by lectin PA-IIL from Pseudomonas aeruginosa: thermodynamics data correlated with X-ray structures., Sabin C, Mitchell EP, Pokorna M, Gautier C, Utille JP, Wimmerova M, Imberty A, FEBS Lett. 2006 Feb 6;580(3):982-7. Epub 2006 Jan 19. PMID:16438968
Page seeded by OCA on Thu Feb 21 16:40:08 2008
Categories: Pseudomonas aeruginosa | Single protein | Imberty, A. | Mitchell, E P. | Sabin, C D. | Wimmerova, M. | CA | GXL | SO4 | Calcium | Galactose | Lectin
