3rtx

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[[Image:3rtx.png|left|200px]]
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==Crystal structure of mammalian capping enzyme (Mce1) and Pol II CTD complex==
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<StructureSection load='3rtx' size='340' side='right' caption='[[3rtx]], [[Resolution|resolution]] 2.81&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3rtx]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RTX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RTX FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GUN:GUANINE'>GUN</scene><br>
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<tr><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene></td></tr>
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<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1i9s|1i9s]], [[1i9t|1i9t]]</td></tr>
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<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Cap1a, MCE1, Rngtt ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])</td></tr>
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<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/mRNA_guanylyltransferase mRNA guanylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.50 2.7.7.50] </span></td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rtx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rtx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rtx RCSB], [http://www.ebi.ac.uk/pdbsum/3rtx PDBsum]</span></td></tr>
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<table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Physical interaction between the phosphorylated RNA polymerase II carboxyl-terminal domain (CTD) and cellular capping enzymes is required for efficient formation of the 5' mRNA cap, the first modification of nascent mRNA. Here, we report the crystal structure of the RNA guanylyltransferase component of mammalian capping enzyme (Mce) bound to a CTD phosphopeptide. The CTD adopts an extended beta-like conformation that docks Tyr1 and Ser5-PO(4) onto the Mce nucleotidyltransferase domain. Structure-guided mutational analysis verified that the Mce-CTD interface is a tunable determinant of CTD binding and stimulation of guanylyltransferase activity, and of Mce function in vivo. The location and composition of the CTD binding site on mammalian capping enzyme is distinct from that of a yeast capping enzyme that recognizes the same CTD primary structure. Thus, capping enzymes from different taxa have evolved different strategies to read the CTD code.
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Structural insights to how mammalian capping enzyme reads the CTD code.,Ghosh A, Shuman S, Lima CD Mol Cell. 2011 Jul 22;43(2):299-310. Epub 2011 Jun 16. PMID:21683636<ref>PMID:21683636</ref>
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The line below this paragraph, containing "STRUCTURE_3rtx", creates the "Structure Box" on the page.
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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or leave the SCENE parameter empty for the default display.
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{{STRUCTURE_3rtx| PDB=3rtx | SCENE= }}
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===Crystal structure of mammalian capping enzyme (Mce1) and Pol II CTD complex===
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
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<references/>
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The line below this paragraph, {{ABSTRACT_PUBMED_21683636}}, adds the Publication Abstract to the page
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__TOC__
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(as it appears on PubMed at http://www.pubmed.gov), where 21683636 is the PubMed ID number.
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</StructureSection>
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{{ABSTRACT_PUBMED_21683636}}
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==About this Structure==
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[[3rtx]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RTX OCA].
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==Reference==
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<ref group="xtra">PMID:021683636</ref><references group="xtra"/>
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[[Category: Mus musculus]]
[[Category: Mus musculus]]
[[Category: MRNA guanylyltransferase]]
[[Category: MRNA guanylyltransferase]]

Revision as of 04:54, 5 June 2014

Crystal structure of mammalian capping enzyme (Mce1) and Pol II CTD complex

3rtx, resolution 2.81Å

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