2bt3
From Proteopedia
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==Overview== | ==Overview== | ||
| - | Molecular wires comprising a Ru(II)- or Re(I)-complex head group, an | + | Molecular wires comprising a Ru(II)- or Re(I)-complex head group, an aromatic tail group, and an alkane linker reversibly inhibit the activity of the copper amine oxidase from Arthrobacter globiformis (AGAO), with K(i) values between 6 muM and 37 nM. In the crystal structure of a Ru(II)-wire:AGAO conjugate, the wire occupies the AGAO active-site substrate access channel, the trihydroxyphenylalanine quinone cofactor is ordered in the "off-Cu" position with its reactive carbonyl oriented toward the inhibitor, and the "gate" residue, Tyr-296, is in the "open" position. Head groups, tail-group substituents, and linker lengths all influence wire-binding interactions with the enzyme. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Arthrobacter globiformis]] | [[Category: Arthrobacter globiformis]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Contakes, S | + | [[Category: Contakes, S M.]] |
| - | [[Category: Dooley, D | + | [[Category: Dooley, D M.]] |
| - | [[Category: Duff, A | + | [[Category: Duff, A P.]] |
| - | [[Category: Dunn, A | + | [[Category: Dunn, A R.]] |
| - | [[Category: Freeman, H | + | [[Category: Freeman, H C.]] |
| - | [[Category: Gray, H | + | [[Category: Gray, H B.]] |
| - | [[Category: Guss, J | + | [[Category: Guss, J M.]] |
| - | [[Category: Halpern-Manners, N | + | [[Category: Halpern-Manners, N W.]] |
| - | [[Category: Juda, G | + | [[Category: Juda, G A.]] |
| - | [[Category: Langley, D | + | [[Category: Langley, D B.]] |
[[Category: CU]] | [[Category: CU]] | ||
[[Category: GOL]] | [[Category: GOL]] | ||
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[[Category: tpq]] | [[Category: tpq]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:41:20 2008'' |
Revision as of 14:41, 21 February 2008
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AGAO IN COMPLEX WITH RUTHENIUM-C4-WIRE AT 1.73 ANGSTROMS
Overview
Molecular wires comprising a Ru(II)- or Re(I)-complex head group, an aromatic tail group, and an alkane linker reversibly inhibit the activity of the copper amine oxidase from Arthrobacter globiformis (AGAO), with K(i) values between 6 muM and 37 nM. In the crystal structure of a Ru(II)-wire:AGAO conjugate, the wire occupies the AGAO active-site substrate access channel, the trihydroxyphenylalanine quinone cofactor is ordered in the "off-Cu" position with its reactive carbonyl oriented toward the inhibitor, and the "gate" residue, Tyr-296, is in the "open" position. Head groups, tail-group substituents, and linker lengths all influence wire-binding interactions with the enzyme.
About this Structure
2BT3 is a Single protein structure of sequence from Arthrobacter globiformis with , , , and as ligands. Active as Amine oxidase (copper-containing), with EC number 1.4.3.6 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Reversible inhibition of copper amine oxidase activity by channel-blocking ruthenium(II) and rhenium(I) molecular wires., Contakes SM, Juda GA, Langley DB, Halpern-Manners NW, Duff AP, Dunn AR, Gray HB, Dooley DM, Guss JM, Freeman HC, Proc Natl Acad Sci U S A. 2005 Sep 20;102(38):13451-6. Epub 2005 Sep 12. PMID:16157884
Page seeded by OCA on Thu Feb 21 16:41:20 2008
Categories: Amine oxidase (copper-containing) | Arthrobacter globiformis | Single protein | Contakes, S M. | Dooley, D M. | Duff, A P. | Dunn, A R. | Freeman, H C. | Gray, H B. | Guss, J M. | Halpern-Manners, N W. | Juda, G A. | Langley, D B. | CU | GOL | NA | R4A | SO4 | 3d-structure | Amine oxidase | Competitive inhibition | Copper | Copper containing | Oxidoreductase | Quinone | Ruthenium diimine wires | Tpq
