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2c5l
From Proteopedia
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==Overview== | ==Overview== | ||
| - | Ras proteins signal to a number of distinct pathways by interacting with | + | Ras proteins signal to a number of distinct pathways by interacting with diverse effectors. Studies of ras/effector interactions have focused on three classes, Raf kinases, ral guanylnucleotide-exchange factors, and phosphatidylinositol-3-kinases. Here we describe ras interactions with another effector, the recently identified phospholipase C epsilon (PLCepsilon). We solved structures of PLCepsilon RA domains (RA1 and RA2) by NMR and the structure of the RA2/ras complex by X-ray crystallography. Although the similarity between ubiquitin-like folds of RA1 and RA2 proves that they are homologs, only RA2 can bind ras. Some of the features of the RA2/ras interface are unique to PLCepsilon, while the ability to make contacts with both switch I and II regions of ras is shared only with phosphatidylinositol-3-kinase. Studies of PLCepsilon regulation suggest that, in a cellular context, the RA2 domain, in a mode specific to PLCepsilon, has a role in membrane targeting with further regulatory impact on PLC activity. |
==Disease== | ==Disease== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Small monomeric GTPase]] | [[Category: Small monomeric GTPase]] | ||
| - | [[Category: Bunney, T | + | [[Category: Bunney, T D.]] |
[[Category: Katan, M.]] | [[Category: Katan, M.]] | ||
| - | [[Category: Pearl, L | + | [[Category: Pearl, L H.]] |
| - | [[Category: Roe, S | + | [[Category: Roe, S M.]] |
[[Category: GOL]] | [[Category: GOL]] | ||
[[Category: GTP]] | [[Category: GTP]] | ||
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[[Category: ubiquitin superfold]] | [[Category: ubiquitin superfold]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:45:08 2008'' |
Revision as of 14:45, 21 February 2008
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STRUCTURE OF PLC EPSILON RAS ASSOCIATION DOMAIN WITH HRAS
Contents |
Overview
Ras proteins signal to a number of distinct pathways by interacting with diverse effectors. Studies of ras/effector interactions have focused on three classes, Raf kinases, ral guanylnucleotide-exchange factors, and phosphatidylinositol-3-kinases. Here we describe ras interactions with another effector, the recently identified phospholipase C epsilon (PLCepsilon). We solved structures of PLCepsilon RA domains (RA1 and RA2) by NMR and the structure of the RA2/ras complex by X-ray crystallography. Although the similarity between ubiquitin-like folds of RA1 and RA2 proves that they are homologs, only RA2 can bind ras. Some of the features of the RA2/ras interface are unique to PLCepsilon, while the ability to make contacts with both switch I and II regions of ras is shared only with phosphatidylinositol-3-kinase. Studies of PLCepsilon regulation suggest that, in a cellular context, the RA2 domain, in a mode specific to PLCepsilon, has a role in membrane targeting with further regulatory impact on PLC activity.
Disease
Known diseases associated with this structure: Bladder cancer, somatic OMIM:[190020], Costello syndrome OMIM:[190020], Thyroid carcinoma, follicular, somatic OMIM:[190020]
About this Structure
2C5L is a Protein complex structure of sequences from Homo sapiens with , and as ligands. Active as Small monomeric GTPase, with EC number 3.6.5.2 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Structural and mechanistic insights into ras association domains of phospholipase C epsilon., Bunney TD, Harris R, Gandarillas NL, Josephs MB, Roe SM, Sorli SC, Paterson HF, Rodrigues-Lima F, Esposito D, Ponting CP, Gierschik P, Pearl LH, Driscoll PC, Katan M, Mol Cell. 2006 Feb 17;21(4):495-507. PMID:16483931
Page seeded by OCA on Thu Feb 21 16:45:08 2008
Categories: Homo sapiens | Protein complex | Small monomeric GTPase | Bunney, T D. | Katan, M. | Pearl, L H. | Roe, S M. | GOL | GTP | MG | Disease mutation | Gtp-binding | Lipoprotein | Nucleotide-binding | Oncogene | Palmitate | Prenylation | Proto-oncogene | Ras | Signaling protein | Ubiquitin superfold
