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3tek

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[[Image:3tek.png|left|200px]]
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==ThermoDBP: a non-canonical single-stranded DNA binding protein with a novel structure and mechanism==
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<StructureSection load='3tek' size='340' side='right' caption='[[3tek]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3tek]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermoproteus_tenax Thermoproteus tenax]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TEK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TEK FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Ttx1576 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2271 Thermoproteus tenax])</td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tek FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tek OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tek RCSB], [http://www.ebi.ac.uk/pdbsum/3tek PDBsum]</span></td></tr>
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<table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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ssDNA-binding proteins (SSBs) based on the oligonucleotide-binding fold are considered ubiquitous in nature and play a central role in many DNA transactions including replication, recombination, and repair. We demonstrate that the Thermoproteales, a clade of hyperthermophilic Crenarchaea, lack a canonical SSB. Instead, they encode a distinct ssDNA-binding protein that we term "ThermoDBP," exemplified by the protein Ttx1576 from Thermoproteus tenax. ThermoDBP binds specifically to ssDNA with low sequence specificity. The crystal structure of Ttx1576 reveals a unique fold and a mechanism for ssDNA binding, consisting of an extended cleft lined with hydrophobic phenylalanine residues and flanked by basic amino acids. Two ssDNA-binding domains are linked by a coiled-coil leucine zipper. ThermoDBP appears to have displaced the canonical SSB during the diversification of the Thermoproteales, a highly unusual example of the loss of a "ubiquitous" protein during evolution.
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Displacement of the canonical single-stranded DNA-binding protein in the Thermoproteales.,Paytubi S, McMahon SA, Graham S, Liu H, Botting CH, Makarova KS, Koonin EV, Naismith JH, White MF Proc Natl Acad Sci U S A. 2011 Nov 21. PMID:22106294<ref>PMID:22106294</ref>
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The line below this paragraph, containing "STRUCTURE_3tek", creates the "Structure Box" on the page.
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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or leave the SCENE parameter empty for the default display.
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{{STRUCTURE_3tek| PDB=3tek | SCENE= }}
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===ThermoDBP: a non-canonical single-stranded DNA binding protein with a novel structure and mechanism===
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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</div>
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==See Also==
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<!--
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*[[Single-stranded DNA-binding protein|Single-stranded DNA-binding protein]]
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The line below this paragraph, {{ABSTRACT_PUBMED_22106294}}, adds the Publication Abstract to the page
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== References ==
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(as it appears on PubMed at http://www.pubmed.gov), where 22106294 is the PubMed ID number.
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<references/>
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__TOC__
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{{ABSTRACT_PUBMED_22106294}}
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</StructureSection>
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==About this Structure==
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[[3tek]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermoproteus_tenax Thermoproteus tenax]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TEK OCA].
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==Reference==
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<ref group="xtra">PMID:022106294</ref><references group="xtra"/>
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[[Category: Thermoproteus tenax]]
[[Category: Thermoproteus tenax]]
[[Category: Graham, S.]]
[[Category: Graham, S.]]

Revision as of 06:37, 5 June 2014

ThermoDBP: a non-canonical single-stranded DNA binding protein with a novel structure and mechanism

3tek, resolution 2.00Å

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