2lc4

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:2lc4.png|left|200px]]
+
==Solution Structure of PilP from Pseudomonas aeruginosa==
 +
<StructureSection load='2lc4' size='340' side='right' caption='[[2lc4]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
 +
== Structural highlights ==
 +
[[2lc4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LC4 OCA]. <br>
 +
<b>Activity:</b> <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span><br>
 +
== Publication Abstract from PubMed ==
 +
Type IVa pili are bacterial nanomachines required for colonization of surfaces, but little is known about the organization of proteins in this system. The Pseudomonas aeruginosa pilMNOPQ operon encodes five key members of the transenvelope complex facilitating pilus function. While PilQ forms the outer membrane secretin pore, the functions of the inner membrane-associated proteins PilM/N/O/P are less well defined. Structural characterization of a stable C-terminal fragment of PilP (PilP(Delta71) ) by NMR revealed a modified beta-sandwich fold, similar to that of Neisseria meningitidis PilP, although complementation experiments showed that the two proteins are not interchangeable likely due to divergent surface properties. PilP is an inner membrane putative lipoprotein, but mutagenesis of the putative lipobox had no effect on the localization and function of PilP. A larger fragment, PilP(Delta18-6His) , co-purified with a PilN(Delta44) /PilO(Delta51) heterodimer as a stable complex that eluted from a size exclusion chromatography column as a single peak with a molecular weight equivalent to two heterotrimers with 1:1:1 stoichiometry. Although PilO forms both homodimers and PilN-PilO heterodimers, PilP(Delta18-6His) did not interact stably with PilO(Delta51) alone. Together these data demonstrate that PilN/PilO/PilP interact directly to form a stable heterotrimeric complex, explaining the dispensability of PilP's lipid anchor for localization and function.
-
<!--
+
Characterization of the PilN, PilO and PilP type IVa pilus subcomplex.,Tammam S, Sampaleanu LM, Koo J, Sundaram P, Ayers M, Andrew Chong P, Forman-Kay JD, Burrows LL, Howell PL Mol Microbiol. 2011 Dec;82(6):1496-514. doi:, 10.1111/j.1365-2958.2011.07903.x. Epub 2011 Nov 18. PMID:22053789<ref>PMID:22053789</ref>
-
The line below this paragraph, containing "STRUCTURE_2lc4", creates the "Structure Box" on the page.
+
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
-
or leave the SCENE parameter empty for the default display.
+
-
-->
+
-
{{STRUCTURE_2lc4| PDB=2lc4 | SCENE= }}
+
-
===Solution Structure of PilP from Pseudomonas aeruginosa===
+
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-
 
+
== References ==
-
 
+
<references/>
-
<!--
+
__TOC__
-
The line below this paragraph, {{ABSTRACT_PUBMED_22053789}}, adds the Publication Abstract to the page
+
</StructureSection>
-
(as it appears on PubMed at http://www.pubmed.gov), where 22053789 is the PubMed ID number.
+
-
-->
+
-
{{ABSTRACT_PUBMED_22053789}}
+
-
 
+
-
==About this Structure==
+
-
[[2lc4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LC4 OCA].
+
-
 
+
-
==Reference==
+
-
<ref group="xtra">PMID:022053789</ref><references group="xtra"/>
+
[[Category: Pseudomonas aeruginosa]]
[[Category: Pseudomonas aeruginosa]]
[[Category: Chong, P.]]
[[Category: Chong, P.]]

Revision as of 08:37, 30 April 2014

Solution Structure of PilP from Pseudomonas aeruginosa

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2lc4"
Personal tools