2lc8

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[[Image:2lc8.png|left|200px]]
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==Solution structure of the MLV readthrough pseudoknot==
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<StructureSection load='2lc8' size='340' side='right' caption='[[2lc8]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''>
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== Structural highlights ==
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[[2lc8]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LC8 OCA]. <br>
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<b>Activity:</b> <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span><br>
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== Publication Abstract from PubMed ==
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Most retroviruses require translational recoding of a viral messenger RNA stop codon to maintain a precise ratio of structural (Gag) and enzymatic (Pol) proteins during virus assembly. Pol is expressed exclusively as a Gag-Pol fusion either by ribosomal frameshifting or by read-through of the gag stop codon. Both of these mechanisms occur infrequently and only affect 5-10% of translating ribosomes, allowing the virus to maintain the critical Gag to Gag-Pol ratio. Although it is understood that the frequency of the recoding event is regulated by cis RNA motifs, no mechanistic explanation is currently available for how the critical protein ratio is maintained. Here we present the NMR structure of the murine leukaemia virus recoding signal and show that a protonation-dependent switch occurs to induce the active conformation. The equilibrium is such that at physiological pH the active, read-through permissive conformation is populated at approximately 6%: a level that correlates with in vivo protein quantities. The RNA functions by a highly sensitive, chemo-mechanical coupling tuned to ensure an optimal read-through frequency. Similar observations for a frameshifting signal indicate that this novel equilibrium-based mechanism may have a general role in translational recoding.
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An equilibrium-dependent retroviral mRNA switch regulates translational recoding.,Houck-Loomis B, Durney MA, Salguero C, Shankar N, Nagle JM, Goff SP, D'Souza VM Nature. 2011 Nov 27;480(7378):561-4. doi: 10.1038/nature10657. PMID:22121021<ref>PMID:22121021</ref>
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The line below this paragraph, containing "STRUCTURE_2lc8", creates the "Structure Box" on the page.
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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or leave the SCENE parameter empty for the default display.
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{{STRUCTURE_2lc8| PDB=2lc8 | SCENE= }}
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===Solution structure of the MLV readthrough pseudoknot===
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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== References ==
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<references/>
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The line below this paragraph, {{ABSTRACT_PUBMED_22121021}}, adds the Publication Abstract to the page
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</StructureSection>
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(as it appears on PubMed at http://www.pubmed.gov), where 22121021 is the PubMed ID number.
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{{ABSTRACT_PUBMED_22121021}}
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==About this Structure==
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[[2lc8]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LC8 OCA].
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==Reference==
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<ref group="xtra">PMID:022121021</ref><references group="xtra"/>
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[[Category: Durney, M A.]]
[[Category: Durney, M A.]]
[[Category: Houck-Loomis, B.]]
[[Category: Houck-Loomis, B.]]

Revision as of 08:45, 30 April 2014

Solution structure of the MLV readthrough pseudoknot

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