2v6e
From Proteopedia
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==Overview== | ==Overview== | ||
| - | The termini of linear chromosomes are protected by specialized DNA | + | The termini of linear chromosomes are protected by specialized DNA structures known as telomeres that also facilitate the complete replication of DNA ends. The simplest type of telomere is a covalently closed DNA hairpin structure found in linear chromosomes of prokaryotes and viruses. Bidirectional replication of a chromosome with hairpin telomeres produces a catenated circular dimer that is subsequently resolved into unit-length chromosomes by a dedicated DNA cleavage-rejoining enzyme known as a hairpin telomere resolvase (protelomerase). Here we report a crystal structure of the protelomerase TelK from Klebsiella oxytoca phage varphiKO2, in complex with the palindromic target DNA. The structure shows the TelK dimer destabilizes base pairing interactions to promote the refolding of cleaved DNA ends into two hairpin ends. We propose that the hairpinning reaction is made effectively irreversible by a unique protein-induced distortion of the DNA substrate that prevents religation of the cleaved DNA substrate. |
==About this Structure== | ==About this Structure== | ||
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==Reference== | ==Reference== | ||
| - | An | + | An interlocked dimer of the protelomerase TelK distorts DNA structure for the formation of hairpin telomeres., Aihara H, Huang WM, Ellenberger T, Mol Cell. 2007 Sep 21;27(6):901-13. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17889664 17889664] |
[[Category: Klebsiella phage phiko2]] | [[Category: Klebsiella phage phiko2]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Aihara, H.]] | [[Category: Aihara, H.]] | ||
[[Category: Ellenberger, T.]] | [[Category: Ellenberger, T.]] | ||
| - | [[Category: Huang, W | + | [[Category: Huang, W M.]] |
[[Category: VO4]] | [[Category: VO4]] | ||
[[Category: dna distortion]] | [[Category: dna distortion]] | ||
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[[Category: resolvase]] | [[Category: resolvase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:53:34 2008'' |
Revision as of 16:53, 21 February 2008
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PROTELOMERASE TELK COMPLEXED WITH SUBSTRATE DNA
Overview
The termini of linear chromosomes are protected by specialized DNA structures known as telomeres that also facilitate the complete replication of DNA ends. The simplest type of telomere is a covalently closed DNA hairpin structure found in linear chromosomes of prokaryotes and viruses. Bidirectional replication of a chromosome with hairpin telomeres produces a catenated circular dimer that is subsequently resolved into unit-length chromosomes by a dedicated DNA cleavage-rejoining enzyme known as a hairpin telomere resolvase (protelomerase). Here we report a crystal structure of the protelomerase TelK from Klebsiella oxytoca phage varphiKO2, in complex with the palindromic target DNA. The structure shows the TelK dimer destabilizes base pairing interactions to promote the refolding of cleaved DNA ends into two hairpin ends. We propose that the hairpinning reaction is made effectively irreversible by a unique protein-induced distortion of the DNA substrate that prevents religation of the cleaved DNA substrate.
About this Structure
2V6E is a Single protein structure of sequence from Klebsiella phage phiko2 with as ligand. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
An interlocked dimer of the protelomerase TelK distorts DNA structure for the formation of hairpin telomeres., Aihara H, Huang WM, Ellenberger T, Mol Cell. 2007 Sep 21;27(6):901-13. PMID:17889664
Page seeded by OCA on Thu Feb 21 18:53:34 2008
