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3v6t

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[[Image:3v6t.jpg|left|200px]]
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==Crystal structure of the DNA-bound dHax3, a TAL effector, at 1.85 angstrom==
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<StructureSection load='3v6t' size='340' side='right' caption='[[3v6t]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3v6t]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Xanthomonas Xanthomonas]. The December 2014 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''TAL Effectors'' by David Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2014_12 10.2210/rcsb_pdb/mom_2014_12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V6T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3V6T FirstGlance]. <br>
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3v6p|3v6p]]</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3v6t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3v6t OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3v6t RCSB], [http://www.ebi.ac.uk/pdbsum/3v6t PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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TAL effectors, secreted by phytopathogenic bacteria, recognize host DNA sequences through a central domain of tandem repeats. Each repeat comprises 33 to 35 conserved amino acids and targets a specific base pair using two hypervariable residues (known as RVD) at positions 12 and 13. Here, we report the crystal structures of a 11.5-repeat TAL effector in both DNA-free and DNA-bound states. Each TAL repeat comprises two helices connected by a short RVD-containing loop. The 11.5 repeats form a right-handed, super-helical structure that tracks along the sense strand of DNA duplex, with RVDs contacting the major groove. The 12th residue stabilizes the RVD loop, whereas the 13th residue makes a base-specific contact. Understanding DNA recognition by TAL effectors may facilitate rational design of DNA-binding proteins with biotechnological applications.
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Structural Basis for Sequence-Specific Recognition of DNA by TAL Effectors.,Deng D, Yan C, Pan X, Mahfouz M, Wang J, Zhu JK, Shi Y, Yan N Science. 2012 Jan 5. PMID:22223738<ref>PMID:22223738</ref>
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The line below this paragraph, containing "STRUCTURE_3v6t", creates the "Structure Box" on the page.
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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{{STRUCTURE_3v6t| PDB=3v6t | SCENE= }}
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===Crystal structure of the DNA-bound dHax3, a TAL effector, at 1.85 angstrom===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==See Also==
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*[[TAL effector|TAL effector]]
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The line below this paragraph, {{ABSTRACT_PUBMED_22223738}}, adds the Publication Abstract to the page
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== References ==
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(as it appears on PubMed at http://www.pubmed.gov), where 22223738 is the PubMed ID number.
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<references/>
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__TOC__
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{{ABSTRACT_PUBMED_22223738}}
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</StructureSection>
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[[Category: RCSB PDB Molecule of the Month]]
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==About this Structure==
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[[Category: TAL Effectors]]
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[[3v6t]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Xanthomonas Xanthomonas]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V6T OCA].
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==Reference==
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<ref group="xtra">PMID:022223738</ref><references group="xtra"/>
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[[Category: Xanthomonas]]
[[Category: Xanthomonas]]
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[[Category: Deng, D.]]
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[[Category: Deng, D]]
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[[Category: Pan, X J.]]
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[[Category: Pan, X J]]
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[[Category: Shi, Y G.]]
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[[Category: Shi, Y G]]
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[[Category: Wang, J W.]]
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[[Category: Wang, J W]]
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[[Category: Yan, C Y.]]
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[[Category: Yan, C Y]]
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[[Category: Yan, N.]]
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[[Category: Yan, N]]
[[Category: 11 tandem repeat]]
[[Category: 11 tandem repeat]]
[[Category: Dna binding protein-dna complex]]
[[Category: Dna binding protein-dna complex]]
[[Category: Dna specific binding protein]]
[[Category: Dna specific binding protein]]

Revision as of 09:36, 4 January 2015

Crystal structure of the DNA-bound dHax3, a TAL effector, at 1.85 angstrom

3v6t, resolution 1.85Å

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