2rfk
From Proteopedia
(New page: 200px<br /><applet load="2rfk" size="350" color="white" frame="true" align="right" spinBox="true" caption="2rfk" /> ''''''<br /> ==About this Structure== is a [h...) |
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[[Image:2rfk.jpg|left|200px]]<br /><applet load="2rfk" size="350" color="white" frame="true" align="right" spinBox="true" | [[Image:2rfk.jpg|left|200px]]<br /><applet load="2rfk" size="350" color="white" frame="true" align="right" spinBox="true" | ||
| - | caption="2rfk" /> | + | caption="2rfk, resolution 2.87Å" /> |
| - | ''''''<br /> | + | '''Substrate RNA Positioning in the Archaeal H/ACA Ribonucleoprotein Complex'''<br /> |
| + | |||
| + | ==Overview== | ||
| + | The most complex RNA pseudouridylases are H/ACA ribonucleoprotein, particles, which use a guide RNA for substrate capture and four proteins, (Cbf5, Nop10, Gar1 and L7Ae/NHP2) for substrate modification. Here we, report the three-dimensional structure of a catalytically deficient, archaeal enzyme complex (including the guide RNA and three of the four, essential proteins) bound to a substrate RNA. Extensive interactions of, Cbf5 with one guide-substrate helix and a guide RNA stem shape the forked, guide-substrate RNA complex structure and position the substrate in, proximity of the Cbf5 catalytic center. Our structural and complementary, fluorescence analyses also indicate that precise placement of the target, uridine at the active site requires a conformation of the guide-substrate, RNA duplex that is brought about by the previously identified concurrent, interaction of the guide RNA with L7Ae and a composite Cbf5-Nop10 surface, and further identify a residue that is critical in this process. | ||
==About this Structure== | ==About this Structure== | ||
| - | + | 2RFK is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=AC1:Zn+Binding+Site+For+Residue+B+1'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RFK OCA]. | |
| + | |||
| + | ==Reference== | ||
| + | Substrate RNA positioning in the archaeal H/ACA ribonucleoprotein complex., Liang B, Xue S, Terns RM, Terns MP, Li H, Nat Struct Mol Biol. 2007 Dec 2;. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18059286 18059286] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| + | [[Category: Pyrococcus furiosus]] | ||
| + | [[Category: Li, H.]] | ||
| + | [[Category: Liang, B.]] | ||
| + | [[Category: SECSG, Southeast.Collaboratory.for.Structural.Genomics.]] | ||
| + | [[Category: Terns, M.P.]] | ||
| + | [[Category: Terns, R.M.]] | ||
| + | [[Category: Xue, S.]] | ||
| + | [[Category: ZN]] | ||
| + | [[Category: archaeal h/aca ribonucleoprotein complex]] | ||
| + | [[Category: isomerase]] | ||
| + | [[Category: isomerase/rna complex]] | ||
| + | [[Category: protein-rna complex]] | ||
| + | [[Category: ribosome biogenesis]] | ||
| + | [[Category: rrna processing]] | ||
| + | [[Category: secsg]] | ||
| + | [[Category: southeast collaboratory for structural genomics]] | ||
| + | [[Category: structural genomics]] | ||
| + | [[Category: trna processing]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 6 | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 6 17:24:05 2008'' |
Revision as of 15:24, 6 February 2008
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Substrate RNA Positioning in the Archaeal H/ACA Ribonucleoprotein Complex
Overview
The most complex RNA pseudouridylases are H/ACA ribonucleoprotein, particles, which use a guide RNA for substrate capture and four proteins, (Cbf5, Nop10, Gar1 and L7Ae/NHP2) for substrate modification. Here we, report the three-dimensional structure of a catalytically deficient, archaeal enzyme complex (including the guide RNA and three of the four, essential proteins) bound to a substrate RNA. Extensive interactions of, Cbf5 with one guide-substrate helix and a guide RNA stem shape the forked, guide-substrate RNA complex structure and position the substrate in, proximity of the Cbf5 catalytic center. Our structural and complementary, fluorescence analyses also indicate that precise placement of the target, uridine at the active site requires a conformation of the guide-substrate, RNA duplex that is brought about by the previously identified concurrent, interaction of the guide RNA with L7Ae and a composite Cbf5-Nop10 surface, and further identify a residue that is critical in this process.
About this Structure
2RFK is a Protein complex structure of sequences from Pyrococcus furiosus with as ligand. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Substrate RNA positioning in the archaeal H/ACA ribonucleoprotein complex., Liang B, Xue S, Terns RM, Terns MP, Li H, Nat Struct Mol Biol. 2007 Dec 2;. PMID:18059286
Page seeded by OCA on Wed Feb 6 17:24:05 2008
Categories: Protein complex | Pyrococcus furiosus | Li, H. | Liang, B. | SECSG, Southeast.Collaboratory.for.Structural.Genomics. | Terns, M.P. | Terns, R.M. | Xue, S. | ZN | Archaeal h/aca ribonucleoprotein complex | Isomerase | Isomerase/rna complex | Protein-rna complex | Ribosome biogenesis | Rrna processing | Secsg | Southeast collaboratory for structural genomics | Structural genomics | Trna processing
