3fi1

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[[Image:3fi1.png|left|200px]]
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==NhaA dimer model==
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<StructureSection load='3fi1' size='340' side='right' caption='[[3fi1]], [[Resolution|resolution]] 7.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3fi1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FI1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3FI1 FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fi1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fi1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3fi1 RCSB], [http://www.ebi.ac.uk/pdbsum/3fi1 PDBsum]</span></td></tr>
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<table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fi/3fi1_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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NhaA, the main sodium-proton exchanger in the inner membrane of Escherichia coli, regulates the cytosolic concentrations of H and Na. It is inactive at acidic pH, becomes active between pH 6 and pH 7, and reaches maximum activity at pH 8. By cryo-electron microscopy of two-dimensional crystals grown at pH 4 and incubated at higher pH, we identified two sequential conformational changes in the protein in response to pH or substrate ions. The first change is induced by a rise in pH from 6 to 7 and marks the transition from the inactive state to the pH-activated state. pH activation, which precedes the ion-induced conformational change, is accompanied by an overall expansion of the NhaA monomer and a local ordering of the N-terminus. The second conformational change is induced by the substrate ions Na and Li at pH above 7 and involves a 7-A displacement of helix IVp. This movement would cause a charge imbalance at the ion-binding site that may trigger the release of the substrate ion and open a periplasmic exit channel.
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<!--
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Conformations of NhaA, the Na/H exchanger from Escherichia coli, in the pH-activated and ion-translocating states.,Appel M, Hizlan D, Vinothkumar KR, Ziegler C, Kuhlbrandt W J Mol Biol. 2009 Feb 20;386(2):351-65. Epub 2008 Dec 25. PMID:19135453<ref>PMID:19135453</ref>
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The line below this paragraph, containing "STRUCTURE_3fi1", creates the "Structure Box" on the page.
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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or leave the SCENE parameter empty for the default display.
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-->
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{{STRUCTURE_3fi1| PDB=3fi1 | SCENE= }}
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===NhaA dimer model===
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
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<!--
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<references/>
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The line below this paragraph, {{ABSTRACT_PUBMED_19135453}}, adds the Publication Abstract to the page
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__TOC__
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(as it appears on PubMed at http://www.pubmed.gov), where 19135453 is the PubMed ID number.
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</StructureSection>
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-->
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{{ABSTRACT_PUBMED_19135453}}
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==About this Structure==
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[[3fi1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FI1 OCA].
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==Reference==
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<ref group="xtra">PMID:019135453</ref><ref group="xtra">PMID:019396973</ref><references group="xtra"/>
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[[Category: Escherichia coli k-12]]
[[Category: Escherichia coli k-12]]
[[Category: Appel, M.]]
[[Category: Appel, M.]]

Revision as of 09:47, 21 May 2014

NhaA dimer model

3fi1, resolution 7.00Å

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