2p8d

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==Overview==
==Overview==
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Cyclohexene nucleic acids (CeNA), which are characterized by the presence, of a cyclohexene moiety instead of a natural (deoxy)ribose sugar, are, known to increase the thermal and enzymatic stability when incorporated in, RNA oligonucleotides. As it has been demonstrated that even a single, cyclohexenyl nucleoside, when incorporated in an oligonucleotide, can have, a profound effect on the biological activity of the oligonucleotide, further research is warranted to study the complex of such, oligonucleotides with target proteins. In order to analyse the influence, of CeNA residues onto the helix conformation and hydration of natural, nucleic acid structures, a cyclohexenyl-adenine building block (xAr) was, incorporated into the Dickerson sequence CGCGA(xAr)TTCGCG. The crystal, structure of this sequence determined to a resolution of 1.90 A. The, global helix belongs to the B-type family and shows a water spine, which, is partially broken up by the apolar cyclohexene residue. The cyclohexene, ring adopts the (2)E-conformation allowing a better incorporation of the, residue in the dodecamer sequence. The crystal packing is stabilized by, cobalt hexamine residues and belongs to space group P222(1), never before, reported for nucleic acids.
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Cyclohexene nucleic acids (CeNA), which are characterized by the presence of a cyclohexene moiety instead of a natural (deoxy)ribose sugar, are known to increase the thermal and enzymatic stability when incorporated in RNA oligonucleotides. As it has been demonstrated that even a single cyclohexenyl nucleoside, when incorporated in an oligonucleotide, can have a profound effect on the biological activity of the oligonucleotide, further research is warranted to study the complex of such oligonucleotides with target proteins. In order to analyse the influence of CeNA residues onto the helix conformation and hydration of natural nucleic acid structures, a cyclohexenyl-adenine building block (xAr) was incorporated into the Dickerson sequence CGCGA(xAr)TTCGCG. The crystal structure of this sequence determined to a resolution of 1.90 A. The global helix belongs to the B-type family and shows a water spine, which is partially broken up by the apolar cyclohexene residue. The cyclohexene ring adopts the (2)E-conformation allowing a better incorporation of the residue in the dodecamer sequence. The crystal packing is stabilized by cobalt hexamine residues and belongs to space group P222(1), never before reported for nucleic acids.
==About this Structure==
==About this Structure==
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Influence of the incorporation of a cyclohexenyl nucleic acid (CeNA) residue onto the sequence d(CGCGAATTCGCG)., Robeyns K, Herdewijn P, Van Meervelt L, Nucleic Acids Res. 2007 Dec 26;. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18160414 18160414]
Influence of the incorporation of a cyclohexenyl nucleic acid (CeNA) residue onto the sequence d(CGCGAATTCGCG)., Robeyns K, Herdewijn P, Van Meervelt L, Nucleic Acids Res. 2007 Dec 26;. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18160414 18160414]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Meervelt, L.Van.]]
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[[Category: Meervelt, L Van.]]
[[Category: Robeyns, K.]]
[[Category: Robeyns, K.]]
[[Category: NCO]]
[[Category: NCO]]
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[[Category: sugar modification]]
[[Category: sugar modification]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 13 08:14:18 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:26:58 2008''

Revision as of 16:27, 21 February 2008

Image:2p8d.jpg

2p8d, resolution 1.90Å

Drag the structure with the mouse to rotate

The Structure of the Dickerson Sequence with an Incorporated CeNA Residue

Overview

Cyclohexene nucleic acids (CeNA), which are characterized by the presence of a cyclohexene moiety instead of a natural (deoxy)ribose sugar, are known to increase the thermal and enzymatic stability when incorporated in RNA oligonucleotides. As it has been demonstrated that even a single cyclohexenyl nucleoside, when incorporated in an oligonucleotide, can have a profound effect on the biological activity of the oligonucleotide, further research is warranted to study the complex of such oligonucleotides with target proteins. In order to analyse the influence of CeNA residues onto the helix conformation and hydration of natural nucleic acid structures, a cyclohexenyl-adenine building block (xAr) was incorporated into the Dickerson sequence CGCGA(xAr)TTCGCG. The crystal structure of this sequence determined to a resolution of 1.90 A. The global helix belongs to the B-type family and shows a water spine, which is partially broken up by the apolar cyclohexene residue. The cyclohexene ring adopts the (2)E-conformation allowing a better incorporation of the residue in the dodecamer sequence. The crystal packing is stabilized by cobalt hexamine residues and belongs to space group P222(1), never before reported for nucleic acids.

About this Structure

2P8D is a Single protein structure of sequence from [1] with as ligand. Known structural/functional Sites: , , , and . Full crystallographic information is available from OCA.

Reference

Influence of the incorporation of a cyclohexenyl nucleic acid (CeNA) residue onto the sequence d(CGCGAATTCGCG)., Robeyns K, Herdewijn P, Van Meervelt L, Nucleic Acids Res. 2007 Dec 26;. PMID:18160414

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